ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014
Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Progress in Development of Functional Biological and Synthetic Blood Products to Augment Transfusable Blood Supply in Operational Medicine.Bioengineering (Basel, Switzerland) · 2025Review
- An Optimized Human Erythroblast Differentiation System Reveals Cholesterol-Dependency of Robust Production of Cultured Red Blood Cells Ex Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application.Stem cell research & therapy · 2023Review
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- Regulation of eIF4E guides a unique translational program to control erythroid maturation.Science advances · 2022Article
- Induction of enucleation in primary and immortalized erythroid cells.International journal of hematology · 2022Review
- Industrially Compatible Transfusable iPSC-Derived RBCs: Progress, Challenges and Prospective Solutions.International journal of molecular sciences · 2021Review
- Reproducible immortalization of erythroblasts from multiple stem cell sources provides approach for sustainable RBC therapeutics.Molecular therapy. Methods & clinical development · 2021Article
- Article
- Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.Communications biology · 2021Article
- BMI1 enables extensive expansion of functional erythroblasts from human peripheral blood mononuclear cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays.Scientific reports · 2021Article
- Hematopoietic Stem Cells Culture, Expansion and Differentiation: An Insight into Variable and Available Media.International journal of stem cells · 2020Review
- An Overview of Different Strategies to Recreate the Physiological Environment in Experimental Erythropoiesis.International journal of molecular sciences · 2020Review
- Erythropoiesis In Vitro-A Research and Therapeutic Tool in Thalassemia.Journal of clinical medicine · 2019Review
- Differentiation of Baboon (Cells · 2019Article
- Generation of an immortalized erythroid progenitor cell line from peripheral blood: A model system for the functional analysis of Plasmodium spp. invasion.American journal of hematology · 2019Article
- Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector.BMC biotechnology · 2019Article
- Current Advances in Red Blood Cell Generation Using Stem Cells from Diverse Sources.Stem cells international · 2019Review
- High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
There is a constant shortage of red blood cells (RBCs) from sufficiently matched donors for patients who need chronic transfusion. Ex vivo expansion and maturation of human erythroid precursors (erythroblasts) from the patients or optimally matched donors could represent a potential solution. Proliferating erythroblasts can be expanded from umbilical cord blood mononuclear cells (CB MNCs) ex vivo for 10(6)-10(7)-fold (in ~50 days) before proliferation arrest and reaching sufficient number for broad application. Here, we report that ectopic expression of three genetic factors (Sox2, c-Myc, and an shRNA against TP53 gene) associated with iPSC derivation enables CB-derived erythroblasts to undergo extended expansion (~10(68)-fold in ~12 months) in a serum-free culture condition without change of cell identity or function. These expanding erythroblasts maintain immature erythroblast phenotypes and morphology, a normal diploid karyotype and dependence on a specific combination of growth factors for proliferation throughout expansion period. When being switched to a terminal differentiation condition, these immortalized erythroblasts gradually exit cell cycle, decrease cell size, accumulate hemoglobin, condense nuclei and eventually give rise to enucleated hemoglobin-containing erythrocytes that can bind and release oxygen. Our result may ultimately lead to an alternative approach to generate unlimited numbers of RBCs for personalized transfusion medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.