ArticleGenetics2013
Highly improved gene targeting by germline-specific Cas9 expression in Drosophila.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 361 papers.
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Who cites it
361 citing papers in PubMed, 630 citations in OpenAlex.
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- Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026Review
- Fos regulates age-dependent neuroinflammation in a VAPP58S model of amyotrophic lateral sclerosis.Disease models & mechanisms · 2026Article
- Mitochondrial proteases maintain cellular protein homeostasis and tissue integrity.Cell & bioscience · 2026Article
- Ion transport peptide regulates larval body water balance via a receptor guanylyl cyclase iniScience · 2026Article
- An ARVC-5 Drosophila knock-in model reveals new functions of Tmem43 in lipid homeostasis.Biology open · 2026Article
- Mechanistic dissection of BLTP2 targeting to ER-PM contact sites.bioRxiv : the preprint server for biology · 2026Article
- NimA promotes cell adhesion at the blood brain barrier of the Drosophila nervous system.EMBO reports · 2026Article
- Uev1A counteracts oncogeniceLife · 2026Article
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- CRISPR-Cas9-mediated therapeutics: Current clinical trials and therapy approval landscape to treat human diseases.Molecular therapy. Nucleic acids · 2026Review
- Nature Inspired Delivery Vehicles for CRISPR-Based Genome Editing.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
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- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis in Drosophila melanogaster.Insect biochemistry and molecular biology · 2026Article
- Mechanical control of the insect extracellular matrix nanostructure.Science advances · 2026Article
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- Plant resistance: scientific basis and latest research progress.Frontiers in plant science · 2026Review
- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis inbioRxiv : the preprint server for biology · 2025Article
301 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a simple yet extremely efficient platform for systematic gene targeting by the RNA-guided endonuclease Cas9 in Drosophila. The system comprises two transgenic strains: one expressing Cas9 protein from the germline-specific nanos promoter and the other ubiquitously expressing a custom guide RNA (gRNA) that targets a unique site in the genome. The two strains are crossed to form an active Cas9-gRNA complex specifically in germ cells, which cleaves and mutates the target site. We demonstrate rapid generation of mutants in seven neuropeptide and two microRNA genes in which no mutants have been described. Founder animals stably expressing Cas9-gRNA transmitted germline mutations to an average of 60% of their progeny, a dramatic improvement in efficiency over the previous methods based on transient Cas9 expression. Simultaneous cleavage of two sites by co-expression of two gRNAs efficiently induced internal deletion with frequencies of 4.3-23%. Our method is readily scalable to high-throughput gene targeting, thereby accelerating comprehensive functional annotation of the Drosophila genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.