ArticleGenetics2013
New levels of transcriptome complexity at upper thermal limits in wild Drosophila revealed by exon expression analysis.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Structural mechanism of mRNA decoding by mammalian GTPase GTPBP1.Nature communications · 2025Article
- Gene and transposable element expression in response to stress in temperate and tropical populations of Drosophila.Mobile DNA · 2025Article
- Structural mechanism of mRNA decoding by mammalian GTPase GTPBP1.bioRxiv : the preprint server for biology · 2025Article
- Assessing Curcumin Uptake and Clearance and Their Influence on Superoxide Dismutase Activity inBiotech (Basel (Switzerland)) · 2023Article
- Identification and expression analysis of heat shock protein family genes of gall fly (Procecidochares utilis) under temperature stress.Cell stress & chaperones · 2023Article
- Assessing the role of family level variation and heat shock gene expression in the thermal stress response of the mosquitoPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Signaling through the dystrophin glycoprotein complex affects the stress-dependent transcriptome in Drosophila.Disease models & mechanisms · 2023Article
- Identification of Six Small Heat Shock Protein Genes in Ostrinia furnacalis (Lepidoptera: Pyralidae) and Analysis of Their Expression Patterns in Response to Environmental Stressors.Journal of insect science (Online) · 2022Article
- Article
- Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii.PloS one · 2021Article
- Integrating GWAS and Transcriptomics to Identify the Molecular Underpinnings of Thermal Stress Responses inFrontiers in genetics · 2020Article
- Overexpression of isoform B of Dgp-1 gene enhances locomotor activity in senescent Drosophila males and under heat stress.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2019Article
- Genomic changes associated with adaptation to arid environments in cactophilic Drosophila species.BMC genomics · 2019Article
- The Interplay of Temperature and Genotype on Patterns of Alternative Splicing in Drosophila melanogaster.Genetics · 2016Article
- Temperature-Related Reaction Norms of Gene Expression: Regulatory Architecture and Functional Implications.Molecular biology and evolution · 2015Article
- De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil.BMC genomics · 2015Article
- A comparison of the transcriptome of Drosophila melanogaster in response to entomopathogenic fungus, ionizing radiation, starvation and cold shock.BMC genomics · 2015Article
- Biological invasions, climate change and genomics.Evolutionary applications · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the cellular heat-shock response has been a paradigm for studying the impact of thermal stress on RNA metabolism and gene expression, the genome-wide response to thermal stress and its connection to physiological stress resistance remain largely unexplored. Here, we address this issue using an array-based exon expression analysis to interrogate the transcriptome in recently established Drosophila melanogaster stocks during severe thermal stress and recovery. We first demonstrated the efficacy of exon-level analyses to reveal a level of thermally induced transcriptome complexity extending well beyond gene-level analyses. Next, we showed that the upper range of both the cellular and physiological thermal stress response profoundly affected message expression and processing in D. melanogaster, limiting expression to a small subset of transcripts, many that share features of known rapidly responding stress genes. As predicted from cellular heat-shock research, constitutive splicing was blocked in a set of novel genes; we did not detect changes to alternative splicing during heat stress, but rather induction of intronless isoforms of known heat-responsive genes. We observed transcriptome plasticity in the form of differential isoform expression during recovery from heat shock, mediated by multiple mechanisms including alternative transcription and alternative splicing. This affected genes involved in DNA regulation, immune response, and thermotolerance. These patterns highlight the complex nature of innate transcriptome responses under stress and potential for adaptive shifts through plasticity and evolved genetic responses at different hierarchical levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.