ArticleScientific reports2013
Discovery of a divergent HPIV4 from respiratory secretions using second and third generation metagenomic sequencing.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.
- Detection of respiratory viruses directly from clinical samples using next-generation sequencing: A literature review of recent advances and potential for routine clinical use.Reviews in medical virology · 2022Pooled it
- Pooled it
- Whole-Genome Phylogenetic Characterization of Human Parainfluenza Virus Type 4 Circulating in St. Petersburg, Russia.Viruses · 2026Article
- Metavirome Analysis and Identification of Midge-Borne Viruses from Yunnan Province, China, in 2021.Viruses · 2023Article
- High-throughput sequencing technologies in the detection of livestock pathogens, diagnosis, and zoonotic surveillance.Computational and structural biotechnology journal · 2022Review
- Utilizing the VirIdAl Pipeline to Search for Viruses in the Metagenomic Data of Bat Samples.Viruses · 2021Article
- Virome in adult Aedes albopictus captured during different seasons in Guangzhou City, China.Parasites & vectors · 2021Article
- Advances in monitoring soil microbial community dynamic and function.Journal of applied genetics · 2020Review
- Review
- Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples.BMC genomics · 2018Article
- Metagenomic Sequencing From Mosquitoes in China Reveals a Variety of Insect and Human Viruses.Frontiers in cellular and infection microbiology · 2018Article
- Metagenomic Analysis ofFrontiers in cellular and infection microbiology · 2018Article
- Metagenomics and Bioinformatics in Microbial Ecology: Current Status and Beyond.Microbes and environments · 2016Review
- Propionibacterium acnes: Disease-Causing Agent or Common Contaminant? Detection in Diverse Patient Samples by Next-Generation Sequencing.Journal of clinical microbiology · 2016Article
- Viral metagenomics and blood safety.Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine · 2016Review
- Characterizing novel endogenous retroviruses from genetic variation inferred from short sequence reads.Scientific reports · 2015Article
- Investigation of Human Cancers for Retrovirus by Low-Stringency Target Enrichment and High-Throughput Sequencing.Scientific reports · 2015Article
- Deep sequencing: becoming a critical tool in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2014Review
- Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing.BMC infectious diseases · 2014Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 7 institutions in 3 countries.
Funding
Abstract
Molecular detection of viruses has been aided by high-throughput sequencing, permitting the genomic characterization of emerging strains. In this study, we comprehensively screened 500 respiratory secretions from children with upper and/or lower respiratory tract infections for viral pathogens. The viruses detected are described, including a divergent human parainfluenza virus type 4 from GS FLX pyrosequencing of 92 specimens. Complete full-genome characterization of the virus followed, using Single Molecule, Real-Time (SMRT) sequencing. Subsequent "primer walking" combined with Sanger sequencing validated the RS platform's utility in viral sequencing from complex clinical samples. Comparative genomics reveals the divergent strain clusters with the only completely sequenced HPIV4a subtype. However, it also exhibits various structural features present in one of the HPIV4b reference strains, opening questions regarding their lifecycle and evolutionary relationships among these viruses. Clinical data from patients infected with the strain, as well as viral prevalence estimates using real-time PCR, is also described.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.