Evidence map›Paper›PMID 24002328›Full record

ArticlePediatric research2013

Packed red cell transfusions alter mesenteric arterial reactivity and nitric oxide pathway in preterm lambs.

Jayasree Nair, Sylvia F Gugino, Lori C Nielsen, Cheryl Allen, James A Russell, Bobby Mathew, Daniel D Swartz, Satyan Lakshminrusimha

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pediatric research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
  2. Paneth cell ontogeny in term and preterm ovine models.Frontiers in veterinary science · 2024
    Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Necrotizing Enterocolitis in Moderate Preterm Infants.BioMed research international · 2018
    Article
  8. Article
  9. Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jayasree NairDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
Sylvia F GuginoDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
Lori C NielsenDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
Cheryl AllenDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
James A Russell1] Department of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York [2] Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York.
Bobby MathewDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
Daniel D SwartzDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
Satyan LakshminrusimhaDepartment of Pediatrics, Division of Neonatology, State University of New York at Buffalo, Buffalo, New York.
University at Buffalo, State University of New York · US

Funding

Optimal Oxygenation in Neonatal Lung InjuryR01HD072929 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Satyanarayana Lakshminrusimha · 2012 to 2026
$5.4M
NICHD NIH HHS 1R01HD072929-01NICHD NIH HHS R01 HD072929
6 · The paper itself

Abstract

backgroundCases of necrotizing enterocolitis occurring within 48 h of packed red blood cell (PRBC) transfusions are increasingly being described in observational studies. Transfusion-associated gut injury is speculated to result from an abnormal mesenteric vascular response to transfusion. However, the mechanism of disruption of the balance between mesenteric vasoconstriction and relaxation following transfusion is not known.

methodsPreterm lambs (n = 16, 134 d gestation; term: 145-147 d) were delivered and ventilated for 24 h. All the lambs received orogastric feeds with colostrum. In addition, 10 of these lambs received PRBC transfusions. Vasoreactivity was evaluated in isolated mesenteric arterial rings using norepinephrine and endothelin-1 as vasoconstrictors. Endothelium-dependent (A23187, a calcium ionophore) and endothelium-independent (SNAP) nitric oxide (NO) donors were used as vasorelaxants. Mesenteric arterial endothelial NO synthase (eNOS), soluble guanylyl cyclase (sGC), and phosphodiesterase 5 (PDE5) mRNA analyses and protein assays were performed.

resultsTransfusion with PRBC significantly increased mesenteric vasoconstriction to norepinephrine and endothelin-1 and impaired relaxation to A23187 and SNAP. Mesenteric arterial eNOS protein decreased following PRBC transfusion. No significant changes were noted in sGC and PDE5 mRNA or protein assays.

conclusionPRBC transfusion in enterally fed preterm lambs promotes mesenteric vasoconstriction and impairs vasorelaxation by reducing mesenteric arterial eNOS.

Indexed as

Animals, NewbornErythrocyte TransfusionObstetric Labor, PrematureAnimalsFemaleMesenteric ArteriesNitric OxidePregnancySheepNitric Oxide

Identifiers

PMID24002328
PMCPMC4401459
OpenAlexW2044865334

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.