ArticleThe Biochemical journal2013
The synaptotagmin 1 linker may function as an electrostatic zipper that opens for docking but closes for fusion pore opening.
Article in The Biochemical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Membrane Merger Dynamics in Exocytosis and Endocytosis.Advances in experimental medicine and biology · 2026Review
- Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy.Molecular biomedicine · 2022Review
- Membrane Association and Functional Mechanism of Synaptotagmin-1 in Triggering Vesicle Fusion.Biophysical journal · 2020Article
- Phosphatidylinositol 4,5 Bisphosphate Controls the cis and trans Interactions of Synaptotagmin 1.Biophysical journal · 2019Article
- Intrinsically disordered proteins in synaptic vesicle trafficking and release.The Journal of biological chemistry · 2019Review
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- Molecular Mechanisms of Fast Neurotransmitter Release.Annual review of biophysics · 2018Review
- Structural Impact of Phosphorylation and Dielectric Constant Variation on Synaptotagmin's IDR.Biophysical journal · 2018Article
- Article
- Phosphorylation of synaptotagmin-1 controls a post-priming step in PKC-dependent presynaptic plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Calcium binding promotes conformational flexibility of the neuronal Ca(2+) sensor synaptotagmin.Biophysical journal · 2015Article
- Synaptotagmin-1 is an antagonist for Munc18-1 in SNARE zippering.The Journal of biological chemistry · 2015Article
- Transmembrane tethering of synaptotagmin to synaptic vesicles controls multiple modes of neurotransmitter release.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Molecular origins of synaptotagmin 1 activities on vesicle docking and fusion pore opening.Scientific reports · 2015Article
- Reconstituting SNARE-mediated membrane fusion at the single liposome level.Methods in cell biology · 2015Article
- Control of membrane gaps by synaptotagmin-Ca2+ measured with a novel membrane distance ruler.Nature communications · 2014Article
- The juxtamembrane linker of full-length synaptotagmin 1 controls oligomerization and calcium-dependent membrane binding.The Journal of biological chemistry · 2014Article
- Nonaggregated α-synuclein influences SNARE-dependent vesicle docking via membrane binding.Biochemistry · 2014Article
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Authors and funding
5 authors.
Funding
Abstract
Syt1 (synaptotagmin 1), a major Ca2+ sensor for fast neurotransmitter release, contains tandem Ca2+-binding C2 domains (C2AB), a single transmembrane α-helix and a highly charged 60-residue-long linker in between. Using single-vesicle-docking and content-mixing assays we found that the linker region of Syt1 is essential for its two signature functions: Ca2+-independent vesicle docking and Ca2+-dependent fusion pore opening. The linker contains the basic-amino-acid-rich N-terminal region and the acidic-amino-acid-rich C-terminal region. When the charge segregation was disrupted, fusion pore opening was slowed, whereas docking was unchanged. Intramolecular disulfide cross-linking between N- and C-terminal regions of the linker or deletion of 40 residues from the linker reduced docking while enhancing pore opening, although the changes were subtle. EPR analysis showed Ca2+-induced line broadening reflecting a conformational change in the linker region. Thus the results of the present study suggest that the electrostatically bipartite linker region may extend for docking and fold to facilitate pore opening.
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