ArticleActa crystallographica. Section D, Biological crystallography2013
Humidity control and hydrophilic glue coating applied to mounted protein crystals improves X-ray diffraction experiments.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Macromolecular crystallography at SPring-8 and SACLA.Journal of synchrotron radiation · 2025Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- In situ crystal data-collection and ligand-screening system at SPring-8.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method.Acta crystallographica. Section D, Structural biology · 2021Article
- Substrate-recognition mechanism of tomato β-galactosidase 4 using X-ray crystallography and docking simulation.Planta · 2020Article
- Sigmoidally hydrochromic molecular porous crystal with rotatable dendrons.Communications chemistry · 2020Article
- A temperature-controlled cold-gas humidifier and its application to protein crystals with the humid-air and glue-coating method.Journal of applied crystallography · 2019Article
- Article
- A solute-binding protein in the closed conformation induces ATP hydrolysis in a bacterial ATP-binding cassette transporter involved in the import of alginate.The Journal of biological chemistry · 2017Article
- A nanosecond time-resolved XFEL analysis of structural changes associated with CO release from cytochrome c oxidase.Science advances · 2017Article
- Controlled dehydration improves the diffraction quality of two RNA crystals.BMC structural biology · 2016Article
- Molecular Mechanism for Conformational Dynamics of Ras·GTP Elucidated from In-Situ Structural Transition in Crystal.Scientific reports · 2016Article
- High-density grids for efficient data collection from multiple crystals.Acta crystallographica. Section D, Structural biology · 2016Article
- An evaluation of adhesive sample holders for advanced crystallographic experiments.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Improved reproducibility of unit-cell parameters in macromolecular cryocrystallography by limiting dehydration during crystal mounting.Acta crystallographica. Section D, Biological crystallography · 2014Article
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4 authors.
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Abstract
Protein crystals are fragile, and it is sometimes difficult to find conditions suitable for handling and cryocooling the crystals before conducting X-ray diffraction experiments. To overcome this issue, a protein crystal-mounting method has been developed that involves a water-soluble polymer and controlled humid air that can adjust the moisture content of a mounted crystal. By coating crystals with polymer glue and exposing them to controlled humid air, the crystals were stable at room temperature and were cryocooled under optimized humidity. Moreover, the glue-coated crystals reproducibly showed gradual transformations of their lattice constants in response to a change in humidity; thus, using this method, a series of isomorphous crystals can be prepared. This technique is valuable when working on fragile protein crystals, including membrane proteins, and will also be useful for multi-crystal data collection.
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