ArticleActa crystallographica. Section D, Biological crystallography2013
Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- TALENs-an indispensable tool in the era of CRISPR: a mini review.Journal, genetic engineering & biotechnology · 2021Review
- α-Helices in the Type III Secretion Effectors: A Prevalent Feature with Versatile Roles.International journal of molecular sciences · 2021Review
- Context and number of noncanonical repeat variable diresidues impede the design of TALE proteins with improved DNA targeting.Protein science : a publication of the Protein Society · 2020Article
- Fine and Predictable Tuning of TALEN Gene Editing Targeting for Improved T Cell Adoptive Immunotherapy.Molecular therapy. Nucleic acids · 2017Article
- The effect of increasing numbers of repeats on TAL effector DNA binding specificity.Nucleic acids research · 2017Article
- Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy.Scientific reports · 2016Article
- Potential Role of the Last Half Repeat in TAL Effectors Revealed by a Molecular Simulation Study.BioMed research international · 2016Article
- DNA-binding proteins from marine bacteria expand the known sequence diversity of TALE-like repeats.Nucleic acids research · 2015Article
- Context influences on TALE-DNA binding revealed by quantitative profiling.Nature communications · 2015Article
- Optimized tuning of TALEN specificity using non-conventional RVDs.Scientific reports · 2015Article
- Article
- Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes.Current opinion in biotechnology · 2014Review
- Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases.BMC molecular biology · 2014Article
- BuD, a helix-loop-helix DNA-binding domain for genome modification.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Repeat 1 of TAL effectors affects target specificity for the base at position zero.Nucleic acids research · 2014Article
- Comprehensive analysis of the specificity of transcription activator-like effector nucleases.Nucleic acids research · 2014Article
- Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response.BMC genomics · 2014Article
- BurrH: a new modular DNA binding protein for genome engineering.Scientific reports · 2014Article
- Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effectors contain a DNA-binding domain organized in tandem repeats. The repeats include two adjacent residues known as the repeat variable di-residue, which recognize a single base pair, establishing a direct code between the dipeptides and the target DNA. This feature suggests this scaffold as an excellent candidate to generate new protein-DNA specificities for biotechnological applications. Here, the crystal structure of AvrBs3 (residues 152-895, molecular mass 82 kDa) in complex with its target DNA sequence is presented, revealing a new mode of interaction with the initial thymine of the target sequence, together with an analysis of both the binding specificity and the thermodynamic properties of AvrBs3. This study quantifies the affinity and the specificity between AvrBs3 and its target DNA. Moreover, in vitro and in vivo analyses reveal that AvrBs3 does not show a strict nucleotide-binding preference for the nucleotide at the zero position of the DNA, widening the number of possible sequences that could be targeted by this scaffold.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.