ArticleIranian journal of basic medical sciences2013
Expression and Antigenic Evaluation of VacA Antigenic Fragment of Helicobacter Pylori.
Article in Iranian journal of basic medical sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
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- Cloning and expression of the enzymatic region of Streptococcal hyaluronidase.Iranian journal of basic medical sciences · 2014Article
- Seroprevalence of anti-Helicobacter pylori and anti-cytotoxin-associated gene A antibodies among healthy individuals in center of Iran.Iranian journal of basic medical sciences · 2014Article
- Recombinant toxin-coregulated pilus A (TcpA) as a candidate subunit cholera vaccine.Iranian journal of microbiology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledObjective(s) : Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity. MATERIALS AND
methodsThe antigenic region of VacA gene was detected by bioinformatics methods. The polymerase chain reaction method was used to amplify a highly antigenic region of VacA gene from chromosomal DNA of H. pylori. The eluted product was cloned into the prokaryotic expression vector pET32a. The target protein was expressed in the Escherichia coli BL21 (DE3) pLysS. The bacteria including pET32a-VacA plasmids were induced by IPTG. The antigenicity was finally studied by western blotting using sera of 15 H. pylori infected patients after purification.
resultsEnzyme digestion analysis, PCR and DNA sequencing results showed that the target gene was inserted correctly into the recombinant vector. The expressed protein was purified successfully via affinity chromatography. Data indicated that antigenic region of VacA protein from Helicobacter pylori was recognized by all 15 patient's sera. Conclusion : Our data showed that antigenic region of VacA protein can be expressed by in E . co.li. This protein was recognized by sera patients suffering from H. pylori infection. the recombinant protein has similar epitopes and close antigenic properties to the natural form of this antigen. Recombinant antigenic region of VacA protein also seems to be a promising antigen for protective and serologic diagnosis .
Indexed as
Identifiers
23997913PMC3758054W1520036240What OpenQuestion holds
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