ArticleJournal of biomolecular techniques : JBT2013
Optimization of whole-body zebrafish sectioning methods for mass spectrometry imaging.
Article in Journal of biomolecular techniques : JBT, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
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- Virological and immunological responses to raltegravir and dolutegravir in the gut-associated lymphoid tissue of HIV-infected men and women.Antiviral therapy · 2018Trial
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- MALDI-TOF mass spectrometry imaging of sulfatide lipid expression in the CNS of mice with experimental autoimmune encephalomyelitis.Scientific reports · 2026Article
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Innovative Application of a Multifunctional Sucrose-Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Method development for correlating lipid molecular information with anatomy in C. elegans.Scientific reports · 2025Article
- Direct transfer of multicellular tumor spheroids grown in agarose microarrays for high-throughput mass spectrometry imaging analysis.Analytical and bioanalytical chemistry · 2025Article
- Comprehensive High-Spatial-Resolution Imaging Metabolomics Workflow for Heterogeneous Tissues.Analytical chemistry · 2025Article
- MALDI-TOF imaging analysis of benzalkonium chloride penetration in ex vivo human skin.PloS one · 2024Article
- Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.Scientific reports · 2023Article
- Desorption electrospray ionization and matrix-assisted laser desorption/ionization as imaging approaches for biological samples analysis.Analytical and bioanalytical chemistry · 2023Review
- Optimization of Mass Spectrometry Imaging for Drug Metabolism and Distribution Studies in the Zebrafish Larvae Model: A Case Study with the Opioid Antagonist Naloxone.International journal of molecular sciences · 2023Article
- Methodological advancements in organ-specific ectopic lipid quantitative characterization: Effects of high fat diet on muscle and liver intracellular lipids.Molecular metabolism · 2023Article
- Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Lipidomics combined with transcriptomic and mass spectrometry imaging analysis of the Asiatic toad (Bufo gargarizans) during metamorphosis and bufadienolide accumulation.Chinese medicine · 2022Article
- Imaging mass spectrometry reveals complex lipid distributions acrossJournal of mass spectrometry and advances in the clinical lab · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Mass spectrometry imaging (MSI) methods and protocols have become widely adapted to a variety of tissues and species. However, the MSI literature contains minimal information on whole-body cryosection preparation for the zebrafish (ZF; Danio rerio), a model organism routinely used in developmental, toxicity, and carcinogenicity studies. The optimal medium for embedding and cryosectioning a whole organism or soft-tissue specimen for histological examination is a synthetic polymer mixture that is incompatible with MSI as a result of ion suppression. We describe the optimal methods and results for embedding and cryosectioning whole-body ZF for MALDI-MSI. We evaluated 13 distinct embedding media formulations and found a supportive hydrogel with the consistency of cartilage to be the optimal embedding medium. The hydrogel medium does not interfere with MSI data collection, aids in tissue stability, is readily available for purchase, and is easy to prepare and handle during cryosectioning. Additionally, we decreased the matrix cluster interference commonly caused by α-cyano-4-hydroxycinnamic acid by adding ammonium phosphate to the solvent spray solution. The optimized methods developed in our laboratory produced high-quality cryosections, as well as high-quality mass spectral images of sectioned ZF.
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