ArticleMolecular & cellular proteomics : MCP2013
A specific PTPRC/CD45 phosphorylation event governed by stem cell chemokine CXCL12 regulates primitive hematopoietic cell motility.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- Single cell transcriptional analysis of human adenoids identifies molecular features of airway microfold cells.Mucosal immunology · 2025Article
- Hematopoietic stem and progenitor cells confer cross-protective trained immunity in mouse models.iScience · 2023Article
- Identification of prognostic hub genes and therapeutic targets for selenium deficiency in chicks model through transcriptome profiling.Scientific reports · 2023Article
- Intravitreal Pharmacokinetic Study of the Antiangiogenic Glycoprotein Opticin.Molecular pharmaceutics · 2020Article
- Genotoxic stress induces Sca-1-expressing metastatic mammary cancer cells.Molecular oncology · 2018Article
- Bioinformatics and functional analyses of key genes and pathways in human clear cell renal cell carcinoma.Oncology letters · 2018Article
- Proteomic analysis of JAK2V617F-induced changes identifies potential new combinatorial therapeutic approaches.Leukemia · 2017Article
- ERK and AKT phosphorylation status in lung cancer and emphysema using nanocapillary isoelectric focusing.BMJ open respiratory research · 2016Article
- Capillary nano-immunoassays: advancing quantitative proteomics analysis, biomarker assessment, and molecular diagnostics.Journal of translational medicine · 2015Review
- Antibody-based detection of protein phosphorylation status to track the efficacy of novel therapies using nanogram protein quantities from stem cells and cell lines.Nature protocols · 2015Article
- c-Myc plays a key role in TADs-induced apoptosis and cell cycle arrest in human hepatocellular carcinoma cells.American journal of cancer research · 2015Article
- Chemical Proteomic Approaches Targeting Cancer Stem Cells: A Review of Current Literature.Cancer genomics & proteomicsReview
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
CXCL12 governs cellular motility, a process deregulated by hematopoietic stem cell oncogenes such as p210-BCR-ABL. A phosphoproteomics approach to the analysis of a hematopoietic progenitor cell line treated with CXCL12 and the Rac 1 and 2 inhibitor NSC23766 has been employed to objectively discover novel mechanisms for regulation of stem cells in normal and malignant hematopoiesis. The proteomic data sets identified new aspects of CXCL12-mediated signaling and novel features of stem cell regulation. We also identified a novel phosphorylation event in hematopoietic progenitor cells that correlated with motile response and governed by the chemotactic factor CXCL12. The novel phosphorylation site on PTPRC/CD45; a protein tyrosine phosphatase, was validated by raising an antibody to the site and also using a mass spectrometry absolute quantification strategy. Site directed mutagenesis and inhibitor studies demonstrated that this single phosphorylation site governs hematopoietic progenitor cell and lymphoid cell motility, lies downstream from Rac proteins and potentiates Src signaling. We have also demonstrated that PTPRC/CD45 is down-regulated in leukemogenic tyrosine kinase expressing cells. The use of discovery proteomics has enabled further understanding of the regulation of PTPRC/CD45 and its important role in cellular motility in progenitor cells.
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Registered trials
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