ArticleGenesis (New York, N.Y. : 2000)2013
Nkx2.2:Cre knock-in mouse line: a novel tool for pancreas- and CNS-specific gene deletion.
Article in Genesis (New York, N.Y. : 2000), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Decoding gene networks controlling hypothalamic and prethalamic neuron development.Cell reports · 2025Article
- Decoding Gene Networks Controlling Hypothalamic and Prethalamic Neuron Development.bioRxiv : the preprint server for biology · 2025Article
- Range of chromatin accessibility configurations are permissive of GABAergic fate acquisition in developing mouse brain.BMC genomics · 2023Article
- Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain.Genes & development · 2023Article
- Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons.Communications biology · 2021Article
- Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development.Nature communications · 2020Article
- Development of a Novel FIJI-Based Method to Investigate Neuronal Circuitry in Neonatal Mice.Developmental neurobiology · 2018Article
- Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons.Cell reports · 2018Article
- Overexpression of Truncated Human DISC1 Induces Appearance of Hindbrain Oligodendroglia in the Forebrain During Development.Schizophrenia bulletin · 2018Article
- Tectal-derived interneurons contribute to phasic and tonic inhibition in the visual thalamus.Nature communications · 2016Article
- Pancreatic regeneration: basic research and gene regulation.Surgery today · 2016Review
- Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Pancreatic islet cell development and regeneration.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- From classical to current: analyzing peripheral nervous system and spinal cord lineage and fate.Developmental biology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Nkx2.2 is a homeodomain-containing transcriptional regulator necessary for the appropriate differentiation of ventral neuronal populations in the spinal cord and hindbrain, and endocrine cell populations in the pancreas and intestine. In each tissue, Nkx2.2 inactivation leads to reciprocal cell fate alterations. To confirm the cell fate changes are due to respecification of Nkx2.2-expressing progenitors and to provide a novel tool for lineage tracing in the pancreas and CNS, we generated an Nkx2.2:Cre mouse line by knocking in a Cre-EGFP cassette into the Nkx2.2 genomic locus and inactivating endogenous Nkx2.2. The R26R-CAG-LSL-tdTomato reporter was used to monitor the specificity and efficiency of Nkx2.2:Cre activity; the tomato reporter faithfully recapitulated endogenous Nkx2.2 expression and could be detected as early as embryonic day (e) 9.25 in the developing CNS and was initiated shortly thereafter at e9.5 in the pancreas. Lineage analyses in the CNS confirmed the cell populations thought to be derived from Nkx2.2-expressing progenitor domains. Furthermore, lineage studies verified Nkx2.2 expression in the earliest pancreatic progenitors that give rise to all cell types of the pancreas; however they also revealed more robust Cre activity in the dorsal versus ventral pancreas. Thus, the Nkx2.2:Cre line provides a novel tool for gene manipulations in the CNS and pancreas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.