Evidence map›Paper›PMID 23996260›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2013

High temporal resolution imaging reveals endosomal membrane penetration and escape of adenoviruses in real time.

Ruben Martinez, Andrew M Burrage, Christopher M Wiethoff, Harald Wodrich

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Ruben MartinezMicrobiologie Fondamental et Pathogénicité, MFP CNRS UMR 5234, University of Bordeaux SEGALEN, Bordeaux, France.
Andrew M Burrage
Christopher M Wiethoff
Harald Wodrich
Loyola University Medical Center · USCentre National de la Recherche Scientifique · FRUniversité de Bordeaux · FR

Funding

Experimental Immunology Training GrantT32AI007508 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI KNIGHT, KATHERINE L. · 1997 to 2022
$4.0M
Inflammasome activation by a respiratory virusR21AI082430 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI WIETHOFF, CHRISTOPHER M · 2009 to 2010
$433k
NIAID NIH HHS AI007508NIAID NIH HHS AI082430NIAID NIH HHS R21 AI082430NIAID NIH HHS T32 AI007508
6 · The paper itself

Abstract

Imaging host-pathogen interactions in real time can provide significant insight into dynamic processes and provide information about time and space of their occurences. Here, we present detailed experimental instructions on how to image the membrane penetration process of the non-enveloped adenovirus in real time. The system is based on a cell line stably expressing the lectin galectin-3 fused to a fluorophore. Membrane-lytic events during adenovirus cell entry can be monitored by the recruitment of galectin-3 to galactose-containing membrane glycoproteins on the exo-surface of ruptured membranes. The simultaneous use of fluorescently labeled adenoviral capsids allows to image the events in unmatched temporal resolution.

Indexed as

Molecular ImagingAdenoviridaeEndosomesFlow CytometryHumansIntracellular MembranesMicroscopy, Confocal

Identifiers

PMID23996260
PMCPMC4199227
OpenAlexW64028389

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.