ArticleMethods in molecular biology (Clifton, N.J.)2013
A high-throughput yeast two-hybrid protocol to determine virus-host protein interactions.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- 800 million years of co-evolution in the green plant lineage: the case of LEUNIG and SEUSS transcriptional co-regulators.Molecular biology and evolution · 2026Article
- Multilabel prediction of virus target proteins via multimodal graph representation learning.PLoS computational biology · 2026Article
- High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Use of Yeast in Biosensing.Microorganisms · 2022Review
- Article
- Article
- The Herpes Simplex Virus Protein pUL31 Escorts Nucleocapsids to Sites of Nuclear Egress, a Process Coordinated by Its N-Terminal Domain.PLoS pathogens · 2015Article
- HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells.Cell host & microbe · 2014Article
- Computational analysis of virus-host interactomes.Methods in molecular biology (Clifton, N.J.) · 2013Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast two-hybrid (Y2H) system is a powerful method to identify and analyze binary protein interactions. In the field of virology, the Y2H system has significantly increased our knowledge of structure and function of viral proteins by systematically assessing intraviral protein interactions. Several comprehensive approaches to determine virus-host interactions have provided insight into viral strategies to manipulate the host for efficient replication and to escape host-derived countermeasures. To expand our knowledge of intraviral and virus-host protein interactions, we here present a Y2H protocol that is well suited for high-throughput screening. Yeast mating followed by liquid handling in a 96-well format as well as fluorescent readout of the reporter system provides a highly standardized and fully automated screening situation. The protocol can either be applied to screen complex host cDNA libraries or protein pairs arrayed for cross-testing. The ease of use, the cost-effectiveness as well as the robotic handling allows for extensive and multiple rounds of screening providing high coverage of protein-protein interactions. Thus, this protocol represents an improved "deep" screening method for high-throughput Y2H assays.
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