ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2014
Escherichia coli biofilm formation and recurrences of urinary tract infections in children.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Distribution ofAntibiotics (Basel, Switzerland) · 2026Article
- Chronic uropathogenic Escherichia coli infections in adolescence: in vitro analysis of clinical isolates, multidrug resistance, and in vivo hippocampal cognitive impacts.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Analysis of phylogroups, biofilm formation, virulence factors, antibiotic resistance and molecular typing of uropathogenic Escherichia coli strains isolated from patients with recurrent and non-recurrent urinary tract infections.BMC infectious diseases · 2025Article
- Biofilm Formation byMicroorganisms · 2023Article
- Urinary Tract Infections Caused by UropathogenicInternational journal of molecular sciences · 2023Review
- Characterization of unconventional pathogenic Escherichia coli isolated from bloodstream infection: virulence beyond the opportunism.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Urinary microbiota of women with recurrent urinary tract infection: collection and culture methods.International urogynecology journal · 2022Article
- Molecular Epidemiology and Presence of Hybrid PathogenicMicroorganisms · 2022Article
- Relationship Between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance inInfection and drug resistance · 2022Article
- Diffusion-Weighted MRI in the Evaluation of Renal Parenchymal Involvement during Febrile Urinary Tract Infections in Children: Preliminary Data.Journal of clinical medicine · 2021Article
- Combination Therapy to Treat Fungal Biofilm-Based Infections.International journal of molecular sciences · 2020Review
- Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and UropathogenicFrontiers in cellular and infection microbiology · 2020Article
- Association Between Kinetics of Early Biofilm Formation and Clonal Lineage inFrontiers in microbiology · 2019Article
- Characteristics of Dogs with Biofilm-Forming Escherichia Coli Urinary Tract Infections.Journal of veterinary internal medicine · 2018Article
- Antimicrobial misuse in pediatric urinary tract infections: recurrences and renal scarring.Annals of clinical microbiology and antimicrobials · 2018Article
- Comparative Genomics ofFrontiers in microbiology · 2018Article
- Strengths and Limitations of Model Systems for the Study of Urinary Tract Infections and Related Pathologies.Microbiology and molecular biology reviews : MMBR · 2016Review
- Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033.Frontiers in microbiology · 2016Article
- Article
- Molecular characterisation of Escherichia coli isolated from hospitalised children and adults with urinary tract infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been suggested that biofilm formation by uropathogenic Escherichia coli (UPEC) isolates is associated with recurrence and persistence of urinary tract infection (UTI). We compared the in vitro biofilm formation of UPEC isolates from children with acute or recurrent UTI. Employing 206 consecutive clinical UPEC isolates from children with proven UTI, i.e., pyelonephritis (n = 78), recurrent pyelonephritis (n = 10), cystitis (n = 84) or recurrent cystitis (n = 34), we applied 1 % crystal violet staining to polystyrene microtitre plates at 72 h and measured the optical density (OD) values. The method had been validated to measure biofilm formation against confocal laser scanning microscopy and scanning electron microscopy. The OD values were lower in the recurrent cystitis group than in the other groups (mean OD 0.36, SD 0.21 vs mean 0.47, SD 0.36, P = 0.04) and higher in the recurrent pyelonephritis group than in the other groups (mean OD 0.69, SD 0.33 vs mean OD 0.44, SD 0.34, P = 0.006) indicating biofilm formation of strains causing recurrent pyelonephritis. It appears that the properties of UPEC isolates required for effective biofilm growth on an abiotic surface are important for recurrent pyelonephritis, but not for recurrent cystitis. It would be valuable in the future to analyze whether the biofilm properties of E. coli observed in vitro predict a slower clinical response to antimicrobial treatment and increased renal scar formation after UTI.
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