ArticleJournal of bacteriology2013
Phosphate concentration and the putative sensor kinase protein CckA modulate cell lysis and release of the Rhodobacter capsulatus gene transfer agent.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- A bacterial CARD-NLR-like immune system controls the release of gene transfer agents.Nature microbiology · 2026Article
- Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination.Nature communications · 2024Article
- Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales.iScience · 2023Article
- Discovery of an Abundant Viral Genus in Polar Regions through the Isolation and Genomic Characterization of a New Virus againstApplied and environmental microbiology · 2023Article
- Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.PHAGE (New Rochelle, N.Y.) · 2022Article
- DNA Gyrase Inhibitors Increase the Frequency of Bacteriophage-like RcGTA-Mediated Gene Transfer inGenes · 2022Article
- Loss of the Rhodobacter capsulatus Serine Acetyl Transferase Gene,Applied and environmental microbiology · 2022Article
- The archetypal gene transfer agent RcGTA is regulated via direct interaction with the enigmatic RNA polymerase omega subunit.Cell reports · 2022Article
- Gene Transfer Agents in Bacterial Endosymbionts of Microbial Eukaryotes.Genome biology and evolution · 2022Article
- Article
- The CckA-ChpT-CtrA Phosphorelay Controlling Rhodobacter capsulatus Gene Transfer Agent Production Is Bidirectional and Regulated by Cyclic di-GMP.Journal of bacteriology · 2021Article
- Cyclic di-GMP-Mediated Regulation of Gene Transfer and Motility in Rhodobacter capsulatus.Journal of bacteriology · 2020Article
- Gene Transfer Agents in Symbiotic Microbes.Results and problems in cell differentiation · 2020Review
- Induction ofJournal of bacteriology · 2019Article
- Identification of the First Gene Transfer Agent (GTA) Small Terminase inJournal of virology · 2019Article
- Identification and characterization of a direct activator of a gene transfer agent.Nature communications · 2019Article
- Integrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response inFrontiers in microbiology · 2019Article
- The Protease ClpXP and the PAS Domain Protein DivL Regulate CtrA and Gene Transfer Agent Production in Rhodobacter capsulatus.Applied and environmental microbiology · 2018Article
- 100 Days of marine Synechococcus-Ruegeria pomeroyi interaction: A detailed analysis of the exoproteome.Environmental microbiology · 2018Article
- Evolution of Bacterial Gene Transfer Agents.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The gene transfer agent of Rhodobacter capsulatus (RcGTA) is a bacteriophage-like genetic element with the sole known function of horizontal gene transfer. Homologues of RcGTA genes are present in many members of the alphaproteobacteria and may serve an important role in microbial evolution. Transcription of RcGTA genes is induced as cultures enter the stationary phase; however, little is known about cis-active sequences. In this work, we identify the promoter of the first gene in the RcGTA structural gene cluster. Additionally, gene transduction frequency depends on the growth medium, and the reason for this is not known. We report that millimolar concentrations of phosphate posttranslationally inhibit the lysis-dependent release of RcGTA from cells in both a complex medium and a defined medium. Furthermore, we found that cell lysis requires the genes rcc00555 and rcc00556, which were expressed and studied in Escherichia coli to determine their predicted functions as an endolysin and holin, respectively. Production of RcGTA is regulated by host systems, including a putative histidine kinase, CckA, and we found that CckA is required for maximal expression of rcc00555 and for maturation of RcGTA to yield gene transduction-functional particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.