ArticleJournal of bacteriology2013
ppGpp-dependent negative control of DNA replication of Shiga toxin-converting bacteriophages in Escherichia coli.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Phage portal proteins counteract stringent-response-mediated restriction.Nature communications · 2026Article
- The opposite effects of stringent response on phage infection ofmicroLife · 2026Article
- Phytochemicals Controlling EnterohemorrhagicInternational journal of molecular sciences · 2025Review
- How to Tackle Bacteriophages: The Review of Approaches with Mechanistic Insight.International journal of molecular sciences · 2023Review
- Replication Region Analysis Reveals Non-lambdoid Shiga Toxin Converting Bacteriophages.Frontiers in microbiology · 2021Article
- Diversity inFrontiers in microbiology · 2020Review
- Formation of Complexes Between O Proteins and Replication Origin Regions of Shiga Toxin-Converting Bacteriophages.Frontiers in molecular biosciences · 2020Article
- Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates.Frontiers in microbiology · 2020Article
- T7 phage factor required for managing RpoS inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Changes in Environmental Conditions Modify Infection Kinetics of Dairy Phages.Food and environmental virology · 2017Article
- ppGpp and cytotoxicity diversity in Shiga toxin-producing Escherichia coli (STEC) isolates.Epidemiology and infection · 2017Article
- Isothiocyanates as effective agents against enterohemorrhagic Escherichia coli: insight to the mode of action.Scientific reports · 2016Article
- Phage adsorption and lytic propagation in Lactobacillus plantarum: could host cell starvation affect them?BMC microbiology · 2015Article
- UV-Sensitivity of Shiga Toxin-Converting Bacteriophage Virions Φ24B, 933W, P22, P27 and P32.Toxins · 2015Article
- Phenethyl isothiocyanate inhibits shiga toxin production in enterohemorrhagic Escherichia coli by stringent response induction.Antimicrobial agents and chemotherapy · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenicity of enterohemorrhagic Escherichia coli (EHEC) strains depends on the production of Shiga toxins that are encoded on lambdoid prophages. Effective production of these toxins requires prophage induction and subsequent phage replication. Previous reports indicated that lytic development of Shiga toxin-converting bacteriophages is inhibited in amino acid-starved bacteria. However, those studies demonstrated that inhibition of both phage-derived plasmid replication and production of progeny virions occurred during the stringent as well as the relaxed response to amino acid starvation, i.e., in the presence as well as the absence of high levels of ppGpp, an alarmone of the stringent response. Therefore, we asked whether ppGpp influences DNA replication and lytic development of Shiga toxin-converting bacteriophages. Lytic development of 5 such bacteriophages was tested in an E. coli wild-type strain and an isogenic mutant that does not produce ppGpp (ppGpp(0)). In the absence of ppGpp, production of progeny phages was significantly (in the range of an order of magnitude) more efficient than in wild-type cells. Such effects were observed in infected bacteria as well as after prophage induction. All tested bacteriophages formed considerably larger plaques on lawns formed by ppGpp(0) bacteria than on those formed by wild-type E. coli. The efficiency of synthesis of phage DNA and relative amount of lambdoid plasmid DNA were increased in cells devoid of ppGpp relative to bacteria containing a basal level of this nucleotide. We conclude that ppGpp negatively influences the lytic development of Shiga toxin-converting bacteriophages and that phage DNA replication efficiency is limited by the stringent control alarmone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.