ArticleDiabetologia2013
Glucose principally regulates insulin secretion in mouse islets by controlling the numbers of granule fusion events per cell.
Article in Diabetologia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Biphasic glucose-stimulated insulin secretion over decades: a journey from measurements and modeling to mechanistic insights.Life metabolism · 2025Review
- Unveiling islet heterogeneity using an automated microfluidic imaging system.Scientific reports · 2024Article
- Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes.Diabetologia · 2024Article
- Functional characteristics of hub and wave-initiator cells in β cell networks.Biophysical journal · 2023Article
- Exendin-4 affects calcium signalling predominantly during activation and activity of beta cell networks in acute mouse pancreas tissue slices.Frontiers in endocrinology · 2023Article
- Article
- Glucose-Stimulated Calcium Dynamics in Beta Cells From Male C57BL/6J, C57BL/6N, and NMRI Mice: A Comparison of Activation, Activity, and Deactivation Properties in Tissue Slices.Frontiers in endocrinology · 2022Article
- Article
- Structural and functional polarisation of human pancreatic beta cells in islets from organ donors with and without type 2 diabetes.Diabetologia · 2021Article
- ABCA12 regulates insulin secretion from β-cells.EMBO reports · 2020Article
- Differentiation of Human Deceased Donor, Adipose-Derived, Mesenchymal Stem Cells into Functional Beta Cells.Journal of stem cells & regenerative medicine · 2020Article
- Optical tools for understanding the complexity of β-cell signalling and insulin release.Nature reviews. Endocrinology · 2018Review
- Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.Lab on a chip · 2018Article
- The role of beta cell heterogeneity in islet function and insulin release.Journal of molecular endocrinology · 2018Review
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Cell polarity defines three distinct domains in pancreatic β-cells.Journal of cell science · 2017Article
- Changes in beta cell function occur in prediabetes and early disease in the Lepr (db) mouse model of diabetes.Diabetologia · 2016Article
- Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets.Diabetologia · 2015Article
- GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation.The Journal of clinical investigation · 2015Article
- Membrane Potential and Calcium Dynamics in Beta Cells from Mouse Pancreas Tissue Slices: Theory, Experimentation, and Analysis.Sensors (Basel, Switzerland) · 2015Review
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisIn dispersed single beta cells the response of each cell to glucose is heterogeneous. In contrast, within an islet, cell-to-cell communication leads to glucose inducing a more homogeneous response. For example, increases in NAD(P)H and calcium are relatively uniform across the cells of the islet. These data suggest that secretion of insulin from single beta cells within an islet should also be relatively homogeneous. The aim of this study was to test this hypothesis by determining the glucose dependence of single-cell insulin responses within an islet.
methodsTwo-photon microscopy was used to detect the glucose-induced fusion of single insulin granules within beta cells in intact mouse islets.
resultsFirst, we validated our assay and showed that the measures of insulin secretion from whole islets could be explained by the time course and numbers of granule fusion events observed. Subsequent analysis of the patterns of granule fusion showed that cell recruitment is a significant factor, accounting for a fourfold increase from 3 to 20 mmol/l glucose. However, the major factor is the regulation of the numbers of granule fusion events within each cell, which increase ninefold over the range of 3 to 20 mmol/l glucose. Further analysis showed that two types of granule fusion event occur: 'full fusion' and 'kiss and run'. We show that the relative frequency of each type of fusion is independent of glucose concentration and is therefore not a factor in the control of insulin secretion. CONCLUSIONS/
interpretationWithin an islet, glucose exerts its main effect through increasing the numbers of insulin granule fusion events within a cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.