ArticleThe EMBO journal2013
High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Slow RNAPII elongation enhances naive pluripotency rewiring while maintaining high replication fork speed.Science advances · 2026Article
- The double life of mammalian DNA replication origins.Genes & development · 2025Review
- RIF1 regulates early replication timing in murine B cells.Nature communications · 2023Article
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Dimeric G-quadruplex motifs-induced NFRs determine strong replication origins in vertebrates.Nature communications · 2023Article
- The esBAF and ISWI nucleosome remodeling complexes influence occupancy of overlapping dinucleosomes and fragile nucleosomes in murine embryonic stem cells.BMC genomics · 2023Article
- 3D chromatin connectivity underlies replication origin efficiency in mouse embryonic stem cells.Nucleic acids research · 2022Article
- Dynamics of replication origin over-activation.Nature communications · 2021Article
- Read, Write, Adapt: Challenges and Opportunities during Kinetoplastid Genome Replication.Trends in genetics : TIG · 2021Review
- Genome duplication ineLife · 2020Article
- Replication origin location might contribute to genetic variability in Trypanosoma cruzi.BMC genomics · 2020Article
- 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication.Scientific reports · 2019Article
- Differences in firing efficiency, chromatin, and transcription underlie the developmental plasticity of theGenome research · 2019Article
- Evidence for DNA Sequence Encoding of an Accessible Nucleosomal Array across Vertebrates.Biophysical journal · 2018Article
- Chromatin conformation regulates the coordination between DNA replication and transcription.Nature communications · 2018Article
- Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication.The EMBO journal · 2017Article
- Genome-wide Mapping of the Nucleosome Landscape by Micrococcal Nuclease and Chemical Mapping.Trends in genetics : TIG · 2017Review
- Nucleosome fragility is associated with future transcriptional response to developmental cues and stress in C. elegans.Genome research · 2017Article
- Selectivity of ORC binding sites and the relation to replication timing, fragile sites, and deletions in cancers.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- DNA replication origins-where do we begin?Genes & development · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA replication origins are poorly characterized genomic regions that are essential to recruit and position the initiation complex to start DNA synthesis. Despite the lack of specific replicator sequences, initiation of replication does not occur at random sites in the mammalian genome. This has lead to the view that DNA accessibility could be a major determinant of mammalian origins. Here, we performed a high-resolution analysis of nucleosome architecture and initiation sites along several origins of different genomic location and firing efficiencies. We found that mammalian origins are highly variable in nucleosome conformation and initiation patterns. Strikingly, initiation sites at efficient CpG island-associated origins always occur at positions of high-nucleosome occupancy. Origin recognition complex (ORC) binding sites, however, occur at adjacent but distinct positions marked by labile nucleosomes. We also found that initiation profiles mirror nucleosome architecture, both at endogenous origins and at a transgene in a heterologous system. Our studies provide a unique insight into the relationship between chromatin structure and initiation sites in the mammalian genome that has direct implications for how the replication programme can be accommodated to diverse epigenetic scenarios.
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