ArticleNature methods2013
Practical innovations for high-throughput amplicon sequencing.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 479 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
479 citing papers in PubMed.
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- Root anatomical traits modulate the assembly and nitrogen-transformation potential of root-associated microbiomes in a temperate steppe.The New phytologist · 2026Article
- Insights into the food safety implications of a commercialApplied and environmental microbiology · 2026Article
- Decoupling Abundance from Centrality: Sexual Dimorphism and Geographical Structuring in thePathogens (Basel, Switzerland) · 2026Article
- Commensal skin bacteria interact with the innate immune system to promote tail regeneration in Xenopus laevis tadpoles.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Review
- Beyond Dominant Symbionts: Low-Abundance Taxa Govern Microbial Network Topology in Sympatric Ticks.Microbial ecology · 2026Article
- Offensive and Defensive Exploitation of Ants by Termitophagous Spiders (Araneae: Zodariidae).Integrative zoology · 2026Article
- Host-selective PMA-PCR enhances bacterial diversity recovery and lowers detection limits in plant microbiome profiling.FEMS microbiology ecology · 2026Article
- Host Species Mediate Distinct Seed Microbiome Responses to Restoration.Microbial ecology · 2026Article
- Tree tissues and species traits modulate the microbial methane-cycling communities of the tree phyllosphere.Scientific reports · 2026Article
- Chronic exposure to Salton Sea aerosols elicits pulmonary inflammation and shifts in murine lung and fecal microbiome diversity.Research square · 2026Article
- Host genotype Impacts the Assembly and Functional Potential of Bacterial Communities in the Spinach Phyllosphere Within Commercial Organic Farming Systems.Microbial ecology · 2026Article
- Mycorrhizal competition release and microbial dynamics in native and non-nativeApplied and environmental microbiology · 2026Article
- Multiple origins of the apple seed microbiome: disentangling sexual and asexual transmission pathways.Environmental microbiome · 2026Article
- Phosphorus application, nutrient absorption, and endophytic root bacterial communities in maize grown in phosphorus-deficient rocky arid soils, China.Scientific reports · 2026Article
- Isolation of N-Fixing Bacteria from Warm-Season Pasture Grasses and the Evaluation of Nitrogen Effects on the Bacterial Communities Present inMicroorganisms · 2026Article
- The bacteria of a fig community.Microbiology spectrum · 2026Article
419 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide. PCR clamps block amplification of contaminating sequences from a eukaryotic host, thereby substantially enriching microbial sequences without introducing bias.
Indexed as
Identifiers
23995388What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.