ArticleJournal of structural biology2013
Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics.
Article in Journal of structural biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 19 citations in OpenAlex.
- Design of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography.Cell reports methods · 2023Article
- Single-particle cryo-EM: alternative schemes to improve dose efficiency.Journal of synchrotron radiation · 2021Article
- Practical Experience with Hole-Free Phase Plates for Cryo Electron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2016Article
- Zernike phase-contrast electron cryotomography applied to marine cyanobacteria infected with cyanophages.Nature protocols · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Imaging with Zernike phase plates is increasingly being used in cryo-TEM tomography and cryo-EM single-particle applications. However, rapid ageing of the phase plates, together with the cost and effort in producing them, present serious obstacles to widespread adoption. We are experimenting with phase plates based on silicon chips that have thin windows; such phase plates could be mass-produced and made available at moderate cost. The windows are coated with conductive layers to reduce charging, and this considerably extends the useful life of the phase plates compared to traditional pure-carbon phase plates. However, a compromise must be reached between robustness and transmission through the phase-plate film. Details are given on testing phase-plate performance by means of imaging an amorphous thin film and evaluating the power spectra of the images.
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Registered trials
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