ArticleChemistry & biology2013
Probing the selectivity and protein·protein interactions of a nonreducing fungal polyketide synthase using mechanism-based crosslinkers.
Article in Chemistry & biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Photoreactive Pantetheinyl Mimics Reveal Design Limitations for Crypto-Acyl Carrier Proteins.Chembiochem : a European journal of chemical biology · 2026Article
- Engineered Chimeras Unveil Swappable Modular Features of Fatty Acid and Polyketide Synthase Acyl Carrier Proteins.Biochemistry · 2022Article
- Design, Synthesis, and Biophysical Evaluation of Mechanism-Based Probes for Condensation Domains of Nonribosomal Peptide Synthetases.ACS chemical biology · 2020Article
- Colorimetric Assay Reports on Acyl Carrier Protein Interactions.Scientific reports · 2019Article
- Genome mining and biosynthesis of a polyketide from a biofertilizer fungus that can facilitate reductive iron assimilation in plant.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes.Natural product reports · 2018Review
- The architectures of iterative type I PKS and FAS.Natural product reports · 2018Review
- The structural organization of substrate loading in iterative polyketide synthases.Nature chemical biology · 2018Article
- Engineering of the Filamentous FungusFrontiers in microbiology · 2018Review
- In trans hydrolysis of carrier protein-bound acyl intermediates by CitA during citrinin biosynthesis.Chemical communications (Cambridge, England) · 2017Article
- Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase-Carrier Protein Cross-Link.Biochemistry · 2017Article
- Covalent Modifiers: A Chemical Perspective on the Reactivity of α,β-Unsaturated Carbonyls with Thiols via Hetero-Michael Addition Reactions.Journal of medicinal chemistry · 2017Article
- Strategies in megasynthase engineering - fatty acid synthases (FAS) as model proteins.Beilstein journal of organic chemistry · 2017Review
- Structural and Biochemical Analysis of Protein-Protein Interactions Between the Acyl-Carrier Protein and Product Template Domain.Angewandte Chemie (International ed. in English) · 2016Article
- Reprogramming acyl carrier protein interactions of an Acyl-CoA promiscuous trans-acyltransferase.Chemistry & biology · 2014Article
- Resin supported acyl carrier protein labeling strategies.RSC advances · 2014Article
- Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.The Journal of biological chemistryReview
Corrections and comments
- Commented on by
- Erratum issuedHaushalter, Bob [corrected to Haushalter, Robert W]; Vagstad, Anna [corrected to Vagstad, Anna L]
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Protein·protein interactions, which often involve interactions among an acyl carrier protein (ACP) and ACP partner enzymes, are important for coordinating polyketide biosynthesis. However, the nature of such interactions is not well understood, especially in the fungal nonreducing polyketide synthases (NR-PKSs) that biosynthesize toxic and pharmaceutically important polyketides. Here, we employ mechanism-based crosslinkers to successfully probe ACP and ketosynthase (KS) domain interactions in NR-PKSs. We found that crosslinking efficiency is closely correlated with the strength of ACP·KS interactions and that KS demonstrates strong starter unit selectivity. We further identified positively charged surface residues by KS mutagenesis, which mediates key interactions with the negatively charged ACP surface. Such complementary/matching contact pairs can serve as "adapter surfaces" for future efforts to generate new polyketides using NR-PKSs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.