ArticleCytotherapy2013
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Article in Cytotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Comparative flow cytometry immunophenotyping of ASCs expanded in conventional flasks versus automated bioreactors.Frontiers in cell and developmental biology · 2026Article
- The monoculture of cord-blood-derived CD34Stem cell reports · 2022Article
- Hollow-fiber bioreactor production of extracellular vesicles from human bone marrow mesenchymal stromal cells yields nanovesicles that mirrors the immuno-modulatory antigenic signature of the producer cell.Stem cell research & therapy · 2021Article
- Microcarrier Screening and Evaluation for Dynamic Expansion of Human Periosteum-Derived Progenitor Cells in a Xenogeneic Free Medium.Frontiers in bioengineering and biotechnology · 2021Article
- Effects of Physical, Chemical, and Biological Stimulus on h-MSC Expansion and Their Functional Characteristics.Annals of biomedical engineering · 2020Review
- Large-scale expansion and characterization of CD3Journal of translational medicine · 2019Article
- Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect.International journal of molecular sciences · 2019Review
- A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells.Stem cell research & therapy · 2019Article
- Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected byStem cell investigation · 2019Article
- Article
- GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System.Molecular therapy. Methods & clinical development · 2018Article
- Biomanufacturing for clinically advanced cell therapies.Nature biomedical engineering · 2018Review
- Optimizing T Cell Expansion in a Hollow-Fiber Bioreactor.Current stem cell reports · 2018Review
- New Delivery Systems of Stem Cells for Vascular Regeneration in Ischemia.Frontiers in cardiovascular medicine · 2017Review
- Culture expansion of adipose derived stromal cells. A closed automated Quantum Cell Expansion System compared with manual flask-based culture.Journal of translational medicine · 2016Article
- Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow.Oncotarget · 2016Article
- Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors.Biotechnology journal · 2016Article
- Implantation of placenta-derived mesenchymal stem cells accelerates murine dermal wound closure through immunomodulation.American journal of translational research · 2016Article
- Asymmetric aneuploidy in mesenchymal stromal cells detected by in situ karyotyping and fluorescence in situ hybridization: suggestions for reference values for stem cells.Stem cells and development · 2015Article
- Efficient manufacturing of therapeutic mesenchymal stromal cells with the use of the Quantum Cell Expansion System.Cytotherapy · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
BACKGROUND
aimsThe Quantum® Cell Expansion System (Quantum; Terumo BCT, Inc, Lakewood, CO, USA) is a novel hollow fiber-based device that automates and closes the cell culture process, reducing labor intensive tasks such as manual cell culture feeding and harvesting. The manual cell selection and expansion processes for the production of clinical-scale quantities of bone marrow-derived human mesenchymal stromal cells (BM-hMSCs) have been successfully translated onto the Quantum platform previously. The formerly static, manual, in vitro process performed primarily on tissue culture polystyrene substrates may raise the question of whether BM-hMSCs cultured on a hollow fiber platform yields comparable cell quality.
methodsA rigorous battery of assays was used to determine the genetic stability of BM-hMSCs selected and produced with the Quantum. In this study, genetic stability was determined by assessing spectral karyotype, micronucleus formation and tumorigenicity to resolve chromosomal aberrations in the stem cell population. Cell phenotype, adherent growth kinetics and tri-lineage differentiation were also evaluated. HMSC bone marrow aspirates, obtained from three approved donors, were expanded in parallel using T225 culture flasks and the Quantum.
resultsBM-hMSCs harvested from the Quantum demonstrated immunophenotype, morphology and tri-lineage differentiation capacity characteristics consistent with the International Society of Cell Therapy standard for hMSCs. Cell populations showed no malignant neoplastic formation in athymic mice 60 days post-transplant, no clonal chromosomal aberrations were observed and no DNA damage was found as measured by micronucleus formation.
conclusionsQuantum-produced BM-hMSCs are of comparable quality and demonstrate analogous genetic stability to BM-hMSCs cultured on tissue culture polystyrene substrates.
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