ArticleACS chemical biology2013
Direct comparison of a genetically encoded sensor and small molecule indicator: implications for quantification of cytosolic Zn(2+).
Article in ACS chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 90 citations in OpenAlex.
- Article
- New Fluorescent Dye for the Detection of ZnJournal of fluorescence · 2025Article
- Changing paradigms for the micronutrient zinc, a known protein cofactor, as a signal relaying also cellular redox state.Quantitative plant biology · 2025Review
- The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms.Biological trace element research · 2024Article
- Protocol for measuring labile cytosolic ZnSTAR protocols · 2024Article
- High-Performance Genetically Encoded Green Fluorescent Biosensors for Intracellular l-Lactate.ACS central science · 2024Article
- Human cells experience a ZnCell reports · 2023Article
- Article
- Genetically encoded fluorescent sensors for metals in biology.Current opinion in chemical biology · 2023Review
- A genetically encoded fluorescent sensor for manganese(II), engineered from lanmodulin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Characterization of Global Gene Expression, Regulation of Metal Ions, and Infection Outcomes in Immune-Competent 129S6 Mouse Macrophages.Infection and immunity · 2021Article
- ZnProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Combining Active Carbonic Anhydrase with Nanogels: Enzyme Protection and Zinc Sensing.International journal of nanomedicine · 2021Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- ZIGIR, a Granule-Specific ZnCell reports · 2020Article
- Chromatographic detection of low-molecular-mass metal complexes in the cytosol of Saccharomyces cerevisiae.Metallomics : integrated biometal science · 2020Article
- A Guide to Human Zinc Absorption: General Overview and Recent Advances of In Vitro Intestinal Models.Nutrients · 2020Review
- Sub-nanomolar sensitive GZnP3 reveals TRPML1-mediated neuronal ZnNature communications · 2019Article
- Intracellular ZnScientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent sensors are powerful tools for visualizing and quantifying molecules and ions in living cells. A variety of small molecule and genetically encoded sensors have been developed for studying intracellular Zn(2+) homeostasis and signaling, but no direct comparisons exist, making it challenging for researchers to identify the appropriate sensor for a given application. Here we directly compare the widely used small molecule probe FluoZin-3 and a genetically encoded sensor, ZapCY2. We demonstrate that, in contrast to FluoZin-3, ZapCY2 exhibits a well-defined cytosolic localization, provides estimates of Zn(2+) concentration with little variability, does not perturb cytosolic Zn(2+) levels, and exhibits rapid Zn(2+) response dynamics. ZapCY2 was used to measure Zn(2+) concentrations in 5 different cell types, revealing higher cytosolic Zn(2+) levels in prostate cancer cells compared to normal prostate cells (although the total zinc is reduced in prostate cancer cells), suggesting distinct regulatory mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.