ArticleJournal of the American Chemical Society2013
In-situ generation of differential sensors that fingerprint kinases and the cellular response to their expression.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
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- Tailored Supramolecular Cage for Efficient Bio-Labeling.International journal of molecular sciences · 2023Article
- A Cavity-Tailored Metal-Organic Tetrahedral Nanocage and Gas Adsorption Property.Nanomaterials (Basel, Switzerland) · 2022Article
- Synthesis of a Tetrahedral Metal-Organic Supramolecular Cage with Dendritic Carbazole Arms.International journal of molecular sciences · 2022Article
- A facile way to construct sensor array library via supramolecular chemistry for discriminating complex systems.Nature communications · 2022Article
- Pattern-recognition-based Sensor Arrays for Cell Characterization: From Materials and Data Analyses to Biomedical Applications.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2020Review
- High-content and high-throughput identification of macrophage polarization phenotypes.Chemical science · 2020Article
- Guest Binding Drives Host Redistribution in Libraries of CoAngewandte Chemie (International ed. in English) · 2020Article
- Quantification of ERK Kinase Activity in Biological Samples Using Differential Sensing.ACS chemical biology · 2020Article
- Label-Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host-Dye Pairs.ChemistryOpen · 2019Article
- A Panel of Protein Kinase Chemosensors Distinguishes Different Types of Fatty Liver Disease.Biochemistry · 2019Article
- Array-based "Chemical Nose" Sensing in Diagnostics and Drug Discovery.Angewandte Chemie (International ed. in English) · 2019Review
- Supramolecular cages as differential sensors for dicarboxylate anions: guest length sensing using principal component analysis of ESI-MS andChemical science · 2019Article
- Article
- A Multi-Fluorescent DNA/Graphene Oxide Conjugate Sensor for Signature-Based Protein Discrimination.Sensors (Basel, Switzerland) · 2017Article
- Small Molecule-Based Pattern Recognition To Classify RNA Structure.Journal of the American Chemical Society · 2017Article
- Art, auto-mechanics, and supramolecular chemistry. A merging of hobbies and career.Beilstein journal of organic chemistry · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Mitogen-activated protein (MAP) kinases are responsible for many cellular functions, and their malfunction manifests itself in several human diseases. Usually, monitoring the phosphorylation states of MAP kinases in vitro requires the preparation and purification of the proteins or Western blotting. Herein, we report an array sensing approach for the differentiation of MAP kinases and their phosphorylated counterparts in vitro. This technique utilizes a library of differential receptors created in situ containing peptides known for affinity to MAP kinases, and a Zn(II)-dipicolylamine complex that binds phosphate groups on proteins. An indicator-displacement assay signals the binding of the individual receptors to the kinases, while chemometrics is used to create a fingerprint for the kinases and their state of activity. For example, linear discriminant analysis correctly identified kinase activity with a classification accuracy of 97.5% in vitro, while the cellular response to kinase expression was classified with 100% accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.