ArticlePloS one2013
Comparison of the force exerted by hippocampal and DRG growth cones.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 275 citations in OpenAlex.
- Synapses without tension fail to fire in an in vitro network of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mechanotransduction in hippocampal neurons operates under localized low picoNewton forces.iScience · 2022Article
- Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.eLife · 2020Article
- Mechanotransduction in neuronal cell development and functioning.Biophysical reviews · 2019Review
- Molecular basis of the functions of the mammalian neuronal growth cone revealed using new methods.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2019Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Article
- Substrate Deformation Predicts Neuronal Growth Cone Advance.Biophysical journal · 2015Article
- Resveratrol Rescues the Impairments of Hippocampal Neurons Stimulated by Microglial Over-Activation In Vitro.Cellular and molecular neurobiology · 2015Article
- Quantifying mechanical force in axonal growth and guidance.Frontiers in cellular neuroscience · 2015Review
- Load sharing in the growth of bundled biopolymers.New journal of physics · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical properties such as force generation are fundamental for neuronal motility, development and regeneration. We used optical tweezers to compare the force exerted by growth cones (GCs) of neurons from the Peripheral Nervous System (PNS), such as Dorsal Root Ganglia (DRG) neurons, and from the Central Nervous System (CNS) such as hippocampal neurons. Developing GCs from dissociated DRG and hippocampal neurons were obtained from P1-P2 and P10-P12 rats. Comparing their morphology, we observed that the area of GCs of hippocampal neurons was 8-10 µm(2) and did not vary between P1-P2 and P10-P12 rats, but GCs of DRG neurons were larger and their area increased from P1-P2 to P10-P12 by 2-4 times. The force exerted by DRG filopodia was in the order of 1-2 pN and never exceeded 5 pN, while hippocampal filopodia exerted a larger force, often in the order of 5 pN. Hippocampal and DRG lamellipodia exerted lateral forces up to 20 pN, but lamellipodia of DRG neurons could exert a vertical force larger than that of hippocampal neurons. Force-velocity relationships (Fv) in both types of neurons had the same qualitative behaviour, consistent with a common autocatalytic model of force generation. These results indicate that molecular mechanisms of force generation of GC from CNS and PNS neurons are similar but the amplitude of generated force is influenced by their cytoskeletal properties.
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