ArticlePloS one2013
De novo transcriptome of Brassica juncea seed coat and identification of genes for the biosynthesis of flavonoids.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Natural variations inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evolutionary studies of the bHLH transcription factors belonging to MBW complex: their role in seed development.Annals of botany · 2023Review
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- A chromosome-level genome assembly of chia provides insights into high omega-3 content and coat color variation of its seeds.Plant communications · 2022Article
- Multi-omics analysis reveals the mechanism of seed coat color formation in Brassica rapa L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- A Walk Through the Maze of Secondary Metabolism in Orchids: A Transcriptomic Approach.Frontiers in plant science · 2022Review
- Metabolite Profiling and Transcriptome Analysis Provide Insight into Seed Coat Color inInternational journal of molecular sciences · 2021Article
- Frequent gain- and loss-of-function mutations of the BjMYB113 gene accounted for leaf color variation in Brassica juncea.BMC plant biology · 2021Article
- Metabolite Profiling and Transcriptome Analyses Provide Insight Into Phenolic and Flavonoid Biosynthesis in the Nutshell of Macadamia Ternifolia.Frontiers in genetics · 2021Article
- Article
- An extreme-phenotype genome-wide association study identifies candidate cannabinoid pathway genes in Cannabis.Scientific reports · 2020Article
- Transcriptomic Landscape of MedicinalFrontiers in plant science · 2020Review
- Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation inFrontiers in genetics · 2020Article
- Transcriptomic comparison between developing seeds of yellow- and black-seeded Brassica napus reveals that genes influence seed quality.BMC plant biology · 2019Article
- Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons.Molecules (Basel, Switzerland) · 2019Article
- Article
- Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations.Scientific reports · 2018Article
- Proanthocyanidin accumulation and transcriptional responses in the seed coat of cranberry beans (Phaseolus vulgaris L.) with different susceptibility to postharvest darkening.BMC plant biology · 2017Article
- Characteristics of Color Development in Seeds of Brown- and Yellow-Seeded Heading Chinese Cabbage and Molecular Analysis ofFrontiers in plant science · 2017Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Brassica juncea, a worldwide cultivated crop plant, produces seeds of different colors. Seed pigmentation is due to the deposition in endothelial cells of proanthocyanidins (PAs), end products from a branch of flavonoid biosynthetic pathway. To elucidate the gene regulatory network of seed pigmentation in B. juncea, transcriptomes in seed coat of a yellow-seeded inbred line and its brown-seeded near- isogenic line were sequenced using the next-generation sequencing platform Illumina/Solexa and de novo assembled. Over 116 million high-quality reads were assembled into 69,605 unigenes, of which about 71.5% (49,758 unigenes) were aligned to Nr protein database with a cut-off E-value of 10(-5). RPKM analysis showed that the brown-seeded testa up-regulated 802 unigenes and down-regulated 502 unigenes as compared to the yellow-seeded one. Biological pathway analysis revealed the involvement of forty six unigenes in flavonoid biosynthesis. The unigenes encoding dihydroflavonol reductase (DFR), leucoantho-cyanidin dioxygenase (LDOX) and anthocyanidin reductase (ANR) for late flavonoid biosynthesis were not expressed at all or at a very low level in the yellow-seeded testa, which implied that these genes for PAs biosynthesis be associated with seed color of B. juncea, as confirmed by qRT-PCR analysis of these genes. To our knowledge, it is the first time to sequence the transcriptome of seed coat in Brassica juncea. The unigene sequences obtained in this study will not only lay the foundations for insight into the molecular mechanisms underlying seed pigmentation in B.juncea, but also provide the basis for further genomics research on this species or its allies.
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