ArticleNucleic acids research2013
Mutant DnaAs of Escherichia coli that are refractory to negative control.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- The loop domains of the E. coli β sliding clamp are required for Hda-dependent inactivation of DnaA.Genetics · 2026Article
- DNA replication initiation timing is important for maintaining genome integrity.Journal of bacteriology · 2025Article
- Structure and kinase activity of bacterial cell cycle regulator CcrZ.PLoS genetics · 2022Article
- Elevated Levels of the Escherichia coliJournal of bacteriology · 2021Article
- Countermeasures to survive excessive chromosome replication in Escherichia coli.Current genetics · 2018Review
- The DnaA Tale.Frontiers in microbiology · 2018Review
- DnaC, the indispensable companion of DnaB helicase, controls the accessibility of DnaB helicase by primase.The Journal of biological chemistry · 2017Article
- Dynamic assembly of Hda and the sliding clamp in the regulation of replication licensing.Nucleic acids research · 2017Article
- Re-wiring of energy metabolism promotes viability during hyperreplication stress in E. coli.PLoS genetics · 2017Article
- The Escherichia coli Cryptic Prophage Protein YfdR Binds to DnaA and Initiation of Chromosomal Replication Is Inhibited by Overexpression of the Gene Cluster yfdQ-yfdR-yfdS-yfdT.Frontiers in microbiology · 2016Article
- The Arg Fingers of Key DnaA Protomers Are Oriented Inward within the Replication Origin oriC and Stimulate DnaA Subcomplexes in the Initiation Complex.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
DnaA is the initiator of DNA replication in bacteria. A mutant DnaA named DnaAcos is unusual because it is refractory to negative regulation. We developed a genetic method to isolate other mutant DnaAs that circumvent regulation to extend our understanding of mechanisms that control replication initiation. Like DnaAcos, one mutant bearing a tyrosine substitution for histidine 202 (H202Y) withstands the regulation exerted by datA, hda and dnaN (β clamp), and both DnaAcos and H202Y resist inhibition by the Hda-β clamp complex in vitro. Other mutant DnaAs carrying G79D, E244K, V303M or E445K substitutions are either only partially sensitive or refractory to inhibition by the Hda-β clamp complex in vitro but are responsive to hda expression in vivo. All mutant DnaAs remain able to interact directly with Hda. Of interest, both DnaAcos and DnaAE244K bind more avidly to Hda. These mutants, by sequestrating Hda, may limit its availability to regulate other DnaA molecules, which remain active to induce extra rounds of DNA replication. Other evidence suggests that a mutant bearing a V292M substitution hyperinitiates by escaping the effect of an unknown regulatory factor. Together, our results provide new insight into the mechanisms that regulate replication initiation in Escherichia coli.
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