ArticleNucleic acids research2013
Sequence-specific microscopic visualization of DNA methylation status at satellite repeats in individual cell nuclei and chromosomes.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Review
- Comprehensive Analysis of Methylome and Transcriptome to Identify Potential Genes Regulating Porcine Testis Development.International journal of molecular sciences · 2024Article
- In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplification.Histochemistry and cell biology · 2023Article
- In focus in HCB.Histochemistry and cell biology · 2023Article
- Imaging-Based In Situ Analysis of 5-Methylcytosine at Low Repetitive Single Gene Loci with Transcription-Activator-Like Effector Probes.ACS chemical biology · 2023Article
- Interstrand crosslinking oligonucleotides elucidate the effect of metal ions on the methylation status of repetitive DNA elements.Frontiers in chemistry · 2023Article
- Satellite DNAs in Health and Disease.Genes · 2022Review
- Sequence, Chromatin and Evolution of Satellite DNA.International journal of molecular sciences · 2021Review
- Engineered TALE Repeats for Enhanced Imaging-Based Analysis of Cellular 5-Methylcytosine.Chembiochem : a European journal of chemical biology · 2021Article
- Designer Receptors for Nucleotide-Resolution Analysis of Genomic 5-Methylcytosine by Cellular Imaging.Angewandte Chemie (International ed. in English) · 2020Article
- Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids.Applied microscopy · 2019Review
- CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome.The Journal of clinical investigation · 2019Article
- Epigenetic DNA Modifications Are Correlated With B Chromosomes and Sex in the CichlidFrontiers in genetics · 2019Article
- Centromere evolution and CpG methylation during vertebrate speciation.Nature communications · 2017Article
- Modular fluorescence complementation sensors for live cell detection of epigenetic signals at endogenous genomic sites.Nature communications · 2017Article
- Ultrastructural localization of 5-methylcytosine on DNA and RNA.Cellular and molecular life sciences : CMLS · 2017Article
- ZNF555 protein binds to transcriptional activator site of 4qA allele and ANT1: potential implication in Facioscapulohumeral dystrophy.Nucleic acids research · 2015Article
- DNA methylation and gene expression dynamics during spermatogonial stem cell differentiation in the early postnatal mouse testis.BMC genomics · 2015Article
- Combined analysis of DNA methylation and cell cycle in cancer cells.Epigenetics · 2015Article
- Whole-mount MeFISH: a novel technique for simultaneous visualization of specific DNA methylation and protein/RNA expression.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylation-specific fluorescence in situ hybridization (MeFISH) was developed for microscopic visualization of DNA methylation status at specific repeat sequences in individual cells. MeFISH is based on the differential reactivity of 5-methylcytosine and cytosine in target DNA for interstrand complex formation with osmium and bipyridine-containing nucleic acids (ICON). Cell nuclei and chromosomes hybridized with fluorescence-labeled ICON probes for mouse major and minor satellite repeats were treated with osmium for crosslinking. After denaturation, fluorescent signals were retained specifically at satellite repeats in wild-type, but not in DNA methyltransferase triple-knockout (negative control) mouse embryonic stem cells. Moreover, using MeFISH, we successfully detected hypomethylated satellite repeats in cells from patients with immunodeficiency, centromeric instability and facial anomalies syndrome and 5-hydroxymethylated satellite repeats in male germ cells, the latter of which had been considered to be unmethylated based on anti-5-methylcytosine antibody staining. MeFISH will be suitable for a wide range of applications in epigenetics research and medical diagnosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.