ArticleMicrobiology (Reading, England)2013
Haloferax volcanii cells lacking the flagellin FlgA2 are hypermotile.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- CsmR controls both, motility and cell shape, in Haloferax volcanii.PLoS genetics · 2026Article
- Cell surface differences within the genusJournal of bacteriology · 2025Review
- Tissue-like multicellular development triggered by mechanical compression in archaea.Science (New York, N.Y.) · 2025Article
- Tat-fimbriae ("tafi"): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway.iScience · 2025Article
- Rhomboid proteases: key players at the cell surface within haloarchaea.Frontiers in microbiology · 2025Article
- TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii.Molecular microbiology · 2024Article
- A comprehensive history of motility and Archaellation in Archaea.FEMS microbes · 2021Article
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- Interaction of two strongly divergent archaellins stabilizes the structure of the Halorubrum archaellum.MicrobiologyOpen · 2020Article
- The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics.Nature communications · 2020Article
- Haloferax volcanii for biotechnology applications: challenges, current state and perspectives.Applied microbiology and biotechnology · 2020Review
- Salt-dependent regulation of archaellins in Haloarcula marismortui.MicrobiologyOpen · 2019Article
- Limited Cross-Complementation BetweenFrontiers in microbiology · 2019Article
- Structure and function of the archaeal response regulator CheY.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cross-kymography analysis to simultaneously quantify the function and morphology of the archaellum.Biophysics and physicobiology · 2018Review
- Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants.Life (Basel, Switzerland) · 2016Article
- Identification of Haloferax volcanii Pilin N-Glycans with Diverse Roles in Pilus Biosynthesis, Adhesion, and Microcolony Formation.The Journal of biological chemistry · 2016Article
- The archaellum: how Archaea swim.Frontiers in microbiology · 2015Article
- Getting a hold on archaeal type IV pili: an expanding repertoire of cellular appendages implicates complex regulation and diverse functions.Frontiers in microbiology · 2015Article
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4 authors at 2 institutions in 2 countries.
Funding
Abstract
Motility driven by rotational movement of flagella allows bacteria and archaea to seek favourable conditions and escape toxic ones. However, archaeal flagella share structural similarities with bacterial type IV pili rather than bacterial flagella. The Haloferax volcanii genome contains two flagellin genes, flgA1 and flgA2. While FlgA1 has been shown to be a major flagellin, the function of FlgA2 is elusive. In this study, it was determined that although FlgA2 by itself does not confer motility to non-motile ΔflgA1 Hfx. volcanii, a subset of these mutant cells contains a flagellum. Consistent with FlgA2 being assembled into functional flagella, FlgA1 expressed from a plasmid can only complement a ΔflgA1 strain when co-expressed with chromosomal or plasmid-encoded FlgA2. Surprisingly, a mutant strain lacking FlgA2, but expressing chromosomally encoded FlgA1, is hypermotile, a phenotype that is accompanied by an increased number of flagella per cell, as well as an increased flagellum length. Site-directed mutagenesis resulting in early translational termination of flgA2 suggests that the hypermotility of the ΔflgA2 strain is not due to transcriptional regulation. This, and the fact that plasmid-encoded FlgA2 expression in a ΔflgA2 strain does not reduce its hypermotility, suggests a possible regulatory role for FlgA2 that depends on the relative abundance of FlgA1. Taken together, our results indicate that FlgA2 plays both structural and regulatory roles in Hfx. volcanii flagella-dependent motility. Future studies will build upon the data presented here to elucidate the significance of the hypermotility of this ΔflgA2 mutant, and will illuminate the regulation and function of archaeal flagella.
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