Evidence map›Paper›PMID 23989139›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2013

Crystallographic analysis of an RNA polymerase σ-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.

Andrey Feklistov, Seth A Darst

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Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

2 authors.

Andrey FeklistovLaboratory of Molecular Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. afeklistov@rockefeller.edu
Seth A Darst

Funding

Y-FAMILY POLYMERASES AND CO-FACTORS IN TRANSLESION REPLICATIONP41RR015301 · NCRR · CORNELL UNIVERSITY ITHACA · PI EISENBERG, DAVID · 2001 to 2011
$28.2M
E COLI RNA POLYMERASE SUBUNIT--STRUCTURE/FUNCTIONR01GM053759 · NIGMS · ROCKEFELLER UNIVERSITY · PI DARST, SETH A. · 1996 to 2015
$6.9M
NCRR NIH HHS P41 RR015301NCRR NIH HHS RR-15301NIGMS NIH HHS R01 GM053759
6 · The paper itself

Abstract

Structural studies of -10 promoter element recognition by domain 2 of the RNA polymerase σ subunit [Feklistov & Darst (2011), Cell, 147, 1257-1269] reveal an unusual crystal-packing arrangement dominated by G-quartets. The 3'-terminal GGG motif of the oligonucleotide used in crystallization participates in G-quadruplex formation with GGG motifs from symmetry-related complexes. Stacking between neighboring G-quadruplexes results in the formation of pseudo-continuous four-stranded columns running throughout the length of the crystal (G-columns). Here, a new crystal form is presented with a different arrangement of G-columns and it is proposed that the fortuitous finding of G-quartet packing could be useful in engineering crystal contacts in protein-ssDNA complexes.

Indexed as

G-QuadruplexesCrystallography, X-RayDNA-Directed RNA PolymerasesDNA, Single-StrandedGenetic EngineeringModels, MolecularPromoter Regions, GeneticProtein Structure, TertiaryRecombinant ProteinsSigma FactorThermusDNA-Directed RNA PolymerasesDNA, Single-StrandedRecombinant ProteinsSigma Factor-10 promoter elementG-quadruplexprotein–ssDNA complexesRNA polymerase σ subunit

Identifiers

PMID23989139
PMCPMC3758139

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.