ArticleMolecular and cellular biology1990
Exon as well as intron sequences are cis-regulating elements for the mutually exclusive alternative splicing of the beta tropomyosin gene.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- Genome-wide association between branch point properties and alternative splicing.PLoS computational biology · 2010Article
- The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.Molecular and cellular biology · 2006Article
- A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones.Genome biology · 2006Article
- Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene.Genes & development · 1999Article
- An intronic sequence element mediates both activation and repression of rat fibroblast growth factor receptor 2 pre-mRNA splicing.Molecular and cellular biology · 1998Article
- Sequence elements surrounding the acceptor site suppress alternative splicing of the sarco/endoplasmic reticulum Ca2+-ATPase 2 gene transcript.The Biochemical journal · 1997Article
- Functional analysis of the polypyrimidine tract in pre-mRNA splicing.Nucleic acids research · 1997Article
- Trans-acting factors regulate the expression of CD44 splice variants.The EMBO journal · 1996Article
- A complex of nuclear proteins mediates SR protein binding to a purine-rich splicing enhancer.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Exon skipping induced by cold stress in a potato invertase gene transcript.Nucleic acids research · 1996Article
- Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro.Molecular and cellular biology · 1995Article
- Efficient excision of the upstream large intron from P4-generated pre-mRNA of the parvovirus minute virus of mice requires at least one donor and the 3' splice site of the small downstream intron.Journal of virology · 1995Article
- Cooperation of 5' and 3' processing sites as well as intron and exon sequences in calcitonin exon recognition.Nucleic acids research · 1995Article
- SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex.Molecular and cellular biology · 1994Article
- Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping.Molecular and cellular biology · 1994Article
- Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.Nucleic acids research · 1994Article
- Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1.Molecular and cellular biology · 1994Article
- A novel bipartite splicing enhancer modulates the differential processing of the human fibronectin EDA exon.Nucleic acids research · 1994Article
- Polypurine sequences within a downstream exon function as a splicing enhancer.Molecular and cellular biology · 1994Article
- Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA.Molecular and cellular biology · 1994Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The beta tropomyosin gene contains two internal exons which are spliced in a mutually exclusive manner. Exon 6B is specifically included in the mature transcripts expressed in skeletal muscle or cultured myotubes, while exon 6A is a myoblast- or smooth muscle-specific exon. The intron between them, which is never spliced in normal conditions, contains two characteristic features: first, the unusual location of the branch point at position -105 from the acceptor, and second, the presence of a very long pyrimidine stretch upstream of the skeletal muscle exon. In this study we designed a number of sequence modifications to investigate the role of these two elements and of a computer-predicted secondary structure in the mutually exclusive splicing of the two exons. We found that mutations in the skeletal exon as well as in the upstream intron could change in vivo the tissue-specific pattern as well as the mutually exclusive character of the two exons. Our results suggest that the unusual position of the branch point does not prevent the utilization of exon 6B in myoblasts and that the region around the acceptor site of exon 6B and the polypyrimidine tract have an important role in this control. Last, we discuss the possible implications of secondary structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.