Evidence map›Paper›PMID 23988714›Full record

ArticleNanomedicine : nanotechnology, biology, and medicine2014

Non-covalent assembly of meso-tetra-4-pyridyl porphine with single-stranded DNA to form nano-sized complexes with hydrophobicity-dependent DNA release and anti-tumor activity.

Supratim Ghosh, Kamil B Ucer, Ralph D'Agostino, Ken Grant, Joseph Sirintrapun, Michael J Thomas, Roy Hantgan, Manish Bharadwaj, William H Gmeiner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine : nanotechnology, biology, and medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Supratim GhoshProgram in Molecular Genetics, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Kamil B UcerDepartment of Physics, Wake Forest University, Winston-Salem, NC, USA.
Ralph D'AgostinoComprehensive Cancer Center, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Ken GrantDepartment of Pathology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Joseph SirintrapunDepartment of Pathology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Michael J ThomasDepartment of Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Roy HantganDepartment of Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Manish BharadwajDepartment of Genrontology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
William H GmeinerProgram in Molecular Genetics, Wake Forest School of Medicine, Winston-Salem, NC, USA; Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, USA; Comprehensive Cancer Center, Wake Forest School of Medicine, Winston-Salem, NC, USA. Electronic address: bgmeiner@wakehealth.edu.
Wake Forest University · US

Funding

Tumor Tissue CoreP30CA012197 · NCI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI Ruben A. Mesa · 1985 to 2026
$55.4M
NCI NIH HHS P30 CA012197NCI NIH HHS P30CA012197
6 · The paper itself

Abstract

DNA and porphyrin based therapeutics are important for anti-cancer treatment. The present studies demonstrate single-stranded DNA (ssDNA) assembles with meso-tetra-4-pyridyl porphine (MTP) forming porphyrin:DNA nano-complexes (PDN) that are stable in aqueous solution under physiologically relevant conditions and undergo dissociation with DNA release in hydrophobic environments, including cell membranes. PDN formation is DNA-dependent with the ratio of porphyrin:DNA being approximately two DNA nucleobases per porphyrin. PDN produce reactive oxygen species (ROS) in a light-dependent manner under conditions that favor nano-complex dissociation in the presence of hydrophobic solvents. PDN induce light-dependent cytotoxicity in vitro and anti-tumor activity towards bladder cancer xenografts in vivo. Light-dependent, PDN-mediated cell death results from ROS-mediated localized membrane damage due to lipid peroxidation with mass spectrometry indicating the generation of the lipid peroxidation products 9- and 13-hydroxy octadecanoic acid. Our results demonstrate that PDN have properties useful for therapeutic applications, including cancer treatment. FROM THE CLINICAL EDITOR: In this study, porphyrin-DNA nanocomplexes were investigated as anti-cancer therapeutics inducing ROS production in a light-dependent manner. Efficacy is demonstrated in vitro as well as a in a bladder cancer xenograft model.

Indexed as

AnimalsAntineoplastic AgentsCell DeathCell Line, TumorCell MembraneDNA, Single-StrandedEndosomesFemaleHumansHydrophobic and Hydrophilic InteractionsLipid PeroxidationMiceMice, NudeNanomedicineNeoplasmsNeoplasm TransplantationAntineoplastic AgentsDNA, Single-StrandedPhotosensitizing AgentsPorphyrinsReactive Oxygen SpeciesStearic Acidstetra(4-N-methylpyridyl)porphineCancer therapyMulti-modality nanoparticlePhotodynamic therapyPorphyrin: DNA assembly

Identifiers

PMID23988714
PMCPMC3946208
OpenAlexW2026842151

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.