ArticleBiochemistry2013
Localized permeabilization of E. coli membranes by the antimicrobial peptide Cecropin A.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 90 citations in OpenAlex.
- Membrane-Traversing Peptides: Mechanistic Landscapes, Assays, and Applications.Chemical reviews · 2026Review
- A Solvent-Assisted Supported Bilayer QCM-D Platform for Resolving Composition- and Structure-Dependent Antimicrobial Peptide-Membrane Interactions.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Prebiotic β-Strand Peptides May Be a Plausible Solution to the Protocell Permeability Problem.Journal of molecular evolution · 2026Review
- Bactericidal membrane attack complex formation initiates at the new pole of E. coli.EMBO reports · 2026Article
- The Efficacy of Cecropin Against Multidrug-Resistant Bacteria Is Linked to the Destabilization of Outer Membrane Structure LPS of Gram-Negative Bacteria.Probiotics and antimicrobial proteins · 2025Article
- Research Progress on Extracellular Matrix-Based Composite Materials in Antibacterial Field.Biomaterials research · 2025Review
- Identification of Multifunctional Putative Bioactive Peptides in the Insect Model Red Palm Weevil (Biomolecules · 2024Article
- The evolution of antimicrobial peptide resistance in Pseudomonas aeruginosa is severely constrained by random peptide mixtures.PLoS biology · 2024Article
- Time-resolved cell-to-cell heterogeneity ofFrontiers in bioengineering and biotechnology · 2024Article
- Outer Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in LiveBiosensors · 2023Article
- Article
- A Multiplexed Cell-Free Assay to Screen for Antimicrobial Peptides in Double Emulsion Droplets.Angewandte Chemie (International ed. in English) · 2022Article
- Real-Time Fluorescence Microscopy on LivingInternational journal of molecular sciences · 2022Article
- A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli.Bioresources and bioprocessing · 2021Article
- Investigation of morphological changes of HPS membrane caused by cecropin B through scanning electron microscopy and atomic force microscopy.Journal of veterinary science · 2021Article
- Article
- Diverse Impacts on Prokaryotic and Eukaryotic Membrane Activities from Hydrophobic Subunit Variation Among Nylon-3 Copolymers.ACS chemical biology · 2021Article
- More Than a Pore: A Current Perspective on the In Vivo Mode of Action of the Lipopeptide Antibiotic Daptomycin.Antibiotics (Basel, Switzerland) · 2020Review
- Application of Biophysical Techniques to Investigate the Interaction of Antimicrobial Peptides With Bacterial Cells.Frontiers in medical technology · 2020Review
- Spectroscopic and Microscopic Approaches for Investigating the Dynamic Interactions of Anti-microbial Peptides With Membranes and Cells.Frontiers in medical technology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence microscopy enables detailed observation of the effects of the antimicrobial peptide Cecropin A on the outer membrane (OM) and cytoplasmic membrane (CM) of single E. coli cells with subsecond time resolution. Fluorescence from periplasmic GFP decays and cell growth halts when the OM is permeabilized. Fluorescence from the DNA stain Sytox Green rises when the CM is permeabilized and the stain enters the cytoplasm. The initial membrane disruptions are localized and stable. Septating cells are attacked earlier than nonseptating cells, and curved membrane surfaces are attacked in preference to cylindrical surfaces. Below a threshold bulk Cecropin A concentration, permeabilization is not observed over 30 min. Above this threshold, we observe a lag time of several minutes between Cecropin A addition and OM permeabilization and ∼30 s between OM and CM permeabilization. The long lag times and the existence of a threshold concentration for permeabilization suggest a nucleation mechanism. However, the roughly linear dependence of mean lag time on bulk peptide concentration is not easily reconciled with a nucleation step involving simultaneous insertion of multiple peptides into the bilayer. Monte Carlo simulations suggest that within seconds, the OM permeability becomes comparable to that of a pore of 100 nm diameter or of numerous small pores distributed over a similarly large area.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.