ArticleBiotechnology for biofuels2013
Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 158 citations in OpenAlex.
- Coordinating Calvin Cycle and Glycolysis inACS synthetic biology · 2026Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Article
- RuBisCO-based COFrontiers in bioengineering and biotechnology · 2026Article
- Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway.Journal of biological engineering · 2025Article
- Engineering COInternational journal of molecular sciences · 2025Article
- Evolution-assisted engineering ofBiodesign research · 2025Article
- Perspectives on current and future yeast technologies for ethanol-based biofuels and bioproducts.FEMS yeast research · 2025Review
- Advancing yeast metabolism for a sustainable single carbon bioeconomy.FEMS yeast research · 2025Review
- Prospects of formamide as nitrogen source in biotechnological production processes.Applied microbiology and biotechnology · 2024Review
- Introducing carbon assimilation in yeasts using photosynthetic directed endosymbiosis.Nature communications · 2024Article
- Living in mangroves: a syntrophic scenario unveiling a resourceful microbiome.BMC microbiology · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Carbon efficient production of chemicals with yeasts.Yeast (Chichester, England) · 2023Review
- Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae.Bioresources and bioprocessing · 2023Article
- Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes.Biotechnology for biofuels and bioproducts · 2023Article
- Trends in Research and Development for COACS omega · 2023Review
- Optimizing the balance between heterologous acetate- and CO2-reduction pathways in anaerobic cultures of Saccharomyces cerevisiae strains engineered for low-glycerol production.FEMS yeast research · 2023Article
- Flux-Balance Analysis and Mobile CRISPRi-Guided Deletion of a Conditionally Essential Gene inACS synthetic biology · 2022Article
- Harnessing bioengineered microbes as a versatile platform for space nutrition.Nature communications · 2022Review
7 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRedox-cofactor balancing constrains product yields in anaerobic fermentation processes. This challenge is exemplified by the formation of glycerol as major by-product in yeast-based bioethanol production, which is a direct consequence of the need to reoxidize excess NADH and causes a loss of conversion efficiency. Enabling the use of CO2 as electron acceptor for NADH oxidation in heterotrophic microorganisms would increase product yields in industrial biotechnology.
resultsA hitherto unexplored strategy to address this redox challenge is the functional expression in yeast of enzymes from autotrophs, thereby enabling the use of CO2 as electron acceptor for NADH reoxidation. Functional expression of the Calvin cycle enzymes phosphoribulokinase (PRK) and ribulose-1,5-bisphosphate carboxylase (Rubisco) in Saccharomyces cerevisiae led to a 90% reduction of the by-product glycerol and a 10% increase in ethanol production in sugar-limited chemostat cultures on a mixture of glucose and galactose. Co-expression of the Escherichia coli chaperones GroEL and GroES was key to successful expression of CbbM, a form-II Rubisco from the chemolithoautotrophic bacterium Thiobacillus denitrificans in yeast.
conclusionsOur results demonstrate functional expression of Rubisco in a heterotrophic eukaryote and demonstrate how incorporation of CO2 as a co-substrate in metabolic engineering of heterotrophic industrial microorganisms can be used to improve product yields. Rapid advances in molecular biology should allow for rapid insertion of this 4-gene expression cassette in industrial yeast strains to improve production, not only of 1st and 2nd generation ethanol production, but also of other renewable fuels or chemicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.