ArticleJournal of leukocyte biology2013
An essential role for Rab27a GTPase in eosinophil exocytosis.
Article in Journal of leukocyte biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Mechanisms of Eosinophil Degranulation.Cells · 2026Review
- Loss of Rab44 attenuates ovalbumin-induced allergic airway inflammation by modulating immune responses and eosinophil function.Frontiers in immunology · 2026Article
- The HL-60 clone 15 cell line as a model for leukocyte migration-possibilities and limitations.Frontiers in immunology · 2025Article
- The Immunosuppressive Functions of Eosinophils Are Compromised in Patients With Allergic Rhinitis, Particularly Concerning Rab27a Expression.Immunity, inflammation and disease · 2024Article
- Association of eosinophil-derived neurotoxin levels with asthma control status in patients with aspirin-exacerbated respiratory disease.Clinical and translational allergy · 2023Article
- Overlapping Machinery in Lysosome-Related Organelle Trafficking: A Lesson from Rare Multisystem Disorders.Cells · 2022Review
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- The Enigma of Eosinophil Degranulation.International journal of molecular sciences · 2021Review
- Article
- Contemporary understanding of the secretory granules in human eosinophils.Journal of leukocyte biology · 2018Review
- Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.Communications biology · 2018Article
- Rab27a/Slp2-a complex is involved in Schwann cell myelination.Neural regeneration research · 2016Article
- Vesicular trafficking of immune mediators in human eosinophils revealed by immunoelectron microscopy.Experimental cell research · 2016Review
- CD63 is tightly associated with intracellular, secretory events chaperoning piecemeal degranulation and compound exocytosis in human eosinophils.Journal of leukocyte biology · 2016Article
- Rab27A regulates transport of cell surface receptors modulating multinucleation and lysosome-related organelles in osteoclasts.Scientific reports · 2015Article
- A common variant in RAB27A gene is associated with fractional exhaled nitric oxide levels in adults.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2015Article
- Eosinophil cytokines, chemokines, and growth factors: emerging roles in immunity.Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Eosinophil degranulation has been implicated in inflammatory processes associated with allergic asthma. Rab27a, a Rab-related GTPase, is a regulatory intracellular signaling molecule expressed in human eosinophils. We postulated that Rab27a regulates eosinophil degranulation. We investigated the role of Rab27a in eosinophil degranulation within the context of airway inflammation. Rab27a expression and localization in eosinophils were investigated by using subcellular fractionation combined with Western blot analysis, and the results were confirmed by immunofluorescence analysis of Rab27a and the granule membrane marker CD63. To determine the function of eosinophil Rab27a, we used Ashen mice, a strain of Rab27a-deficient animals. Ashen eosinophils were tested for degranulation in response to PAF and calcium ionophore by measuring released EPX activity. Airway EPX release was also determined by intratracheal injection of eosinophils into mice lacking EPX. Rab27a immunoreactivity colocalized with eosinophil crystalloid granules, as determined by subcellular fractionation and immunofluorescence analysis. PAF induced eosinophil degranulation in correlation with redistribution of Rab27a(+) structures, some of which colocalized with CD63(+) crystalloid granules at the cell membrane. Eosinophils from mice had significantly reduced EPX release compared with normal WT eosinophils, both in vitro and in vivo. In mouse models, Ashen mice demonstrated reduced EPX release in BAL fluid. These findings suggest that Rab27a has a key role in eosinophil degranulation. Furthermore, these findings have implications for Rab27a-dependent eosinophil degranulation in airway inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.