ArticleThe Journal of biological chemistry2013
Control of DNA replication by the nucleus/cytoplasm ratio in Xenopus.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Identification of Critical Molecular Pathways Induced by HDAC11 Overexpression in Cardiac Mesenchymal Stem Cells.Biomolecules · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Distinct signaling signatures drive compensatory proliferation via S-phase acceleration.PLoS genetics · 2022Article
- The Nuclear-to-Cytoplasmic Ratio: Coupling DNA Content to Cell Size, Cell Cycle, and Biosynthetic Capacity.Annual review of genetics · 2022Review
- Temporal Gradients Controlling Embryonic Cell Cycle.Biology · 2021Review
- Polo-like kinase 1 (Plk1) is a positive regulator of DNA replication in theCell cycle (Georgetown, Tex.) · 2020Article
- Genome wide decrease of DNA replication eye density at the midblastula transition ofCell cycle (Georgetown, Tex.) · 2019Article
- On the Interplay of the DNA Replication Program and the Intra-S Phase Checkpoint Pathway.Genes · 2019Review
- ELUCIDATING NUCLEAR SIZE CONTROL IN THE XENOPUS MODEL SYSTEM.Veterinarski glasnik · 2018Article
- Zygotic Genome Activation in Vertebrates.Developmental cell · 2017Review
- Both Nuclear Size and DNA Amount Contribute to Midblastula Transition Timing in Xenopus laevis.Scientific reports · 2017Article
- Recent advances in understanding nuclear size and shape.Nucleus (Austin, Tex.) · 2016Review
- Cell-Size Control.Cold Spring Harbor perspectives in biology · 2016Review
- Use of Xenopus cell-free extracts to study size regulation of subcellular structures.The International journal of developmental biology · 2016Review
- Cell cycle control in the early embryonic development of aquatic animal species.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2015Review
- Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Interaction of Chk1 with Treslin negatively regulates the initiation of chromosomal DNA replication.Molecular cell · 2015Article
- Nuclear size scaling during Xenopus early development contributes to midblastula transition timing.Current biology : CB · 2015Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The nucleus/cytoplasm (N/C) ratio controls S phase dynamics in many biological systems, most notably the abrupt remodeling of the cell cycle that occurs at the midblastula transition in early Xenopus laevis embryos. After an initial series of rapid cleavage cycles consisting only of S and M phases, a critical N/C ratio is reached, which causes a sharp increase in the length of S phase as the cell cycle is reconfigured to resemble somatic cell cycles. How the N/C ratio determines the length of S phase has been a longstanding problem in developmental biology. Using Xenopus egg extracts, we show that DNA replication at high N/C ratio is restricted by one or more limiting substances. We report here that the protein phosphatase PP2A, in conjunction with its B55α regulatory subunit, becomes limiting for replication origin firing at high N/C ratio, and this in turn leads to reduced origin activation and an increase in the time required to complete S phase. Increasing the levels of PP2A catalytic subunit or B55α experimentally restores rapid DNA synthesis at high N/C ratio. Inversely, reduction of PP2A or B55α levels sharply extends S phase even in low N/C extracts. These results identify PP2A-B55α as a link between DNA replication and N/C ratio in egg extracts and suggest a mechanism that may influence the onset of the midblastula transition in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.