ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2013
Hemoglobin isoform differentiation and allosteric regulation of oxygen binding in the turtle, Trachemys scripta.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Global Environmental Factors Impact the Evolution of Adult Hemoglobins in Squamata Reptiles (Lizards and Snakes) and Terrestrial Turtles.Genome biology and evolution · 2025Article
- Convergent High OAnimals : an open access journal from MDPI · 2024Article
- Evolution and molecular basis of a novel allosteric property of crocodilian hemoglobin.Current biology : CB · 2023Article
- New insights into survival strategies to oxygen deprivation in anoxia-tolerant vertebrates.Acta physiologica (Oxford, England) · 2022Review
- Comparison of hematological traits and oxygenation properties of hemoglobins from highland and lowland Asiatic toad (Bufo gargarizans).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Review
- Recent genome duplications facilitate the phenotypic diversity of Hb repertoire in the Cyprinidae.Science China. Life sciences · 2021Article
- Structure and function of crocodilian hemoglobins and allosteric regulation by chloride, ATP, and COAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles.The Journal of experimental biology · 2019Article
- Allosteric mechanisms underlying the adaptive increase in hemoglobin-oxygen affinity of the bar-headed goose.The Journal of experimental biology · 2018Article
- Gene Turnover and Diversification of the α- and β-Globin Gene Families in Sauropsid Vertebrates.Genome biology and evolution · 2018Article
- Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish.Scientific reports · 2017Article
- Regulation of blood oxygen transport in hibernating mammals.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
- The Greenland shark Somniosus microcephalus-Hemoglobins and ligand-binding properties.PloS one · 2017Article
- Hemoglobin-oxygen affinity in high-altitude vertebrates: is there evidence for an adaptive trend?The Journal of experimental biology · 2016Review
- Gene Duplication and Evolutionary Innovations in Hemoglobin-Oxygen Transport.Physiology (Bethesda, Md.) · 2016Review
- Oxygenation properties and isoform diversity of snake hemoglobins.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Genetically based low oxygen affinities of felid hemoglobins: lack of biochemical adaptation to high-altitude hypoxia in the snow leopard.The Journal of experimental biology · 2015Article
- Gene turnover in the avian globin gene families and evolutionary changes in hemoglobin isoform expression.Molecular biology and evolution · 2015Article
- Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria.PloS one · 2015Article
- Differences in Hematological Traits between High- and Low-Altitude Lizards (Genus Phrynocephalus).PloS one · 2015Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
When freshwater turtles acclimatize to winter hibernation, there is a gradual transition from aerobic to anaerobic metabolism, which may require adjustments of blood O2 transport before turtles become anoxic. Here, we report the effects of protons, anionic cofactors, and temperature on the O2-binding properties of isolated hemoglobin (Hb) isoforms, HbA and HbD, in the turtle Trachemys scripta. We determined the primary structures of the constituent subunits of the two Hb isoforms, and we related the measured functional properties to differences in O2 affinity between untreated hemolysates from turtles that were acclimated to normoxia and anoxia. Our data show that HbD has a consistently higher O2 affinity compared with HbA, whereas Bohr and temperature effects, as well as thiol reactivity, are similar. Although sequence data show amino acid substitutions at two known β-chain ATP-binding site positions, we find high ATP affinities for both Hb isoforms, suggesting an alternative and stronger binding site for ATP. The high ATP affinities indicate that, although ATP levels decrease in red blood cells of turtles acclimating to anoxia, the O2 affinity would remain largely unchanged, as confirmed by O2-binding measurements of untreated hemolysates from normoxic and anoxic turtles. Thus, the increase in blood-O2 affinity that accompanies winter acclimation is mainly attributable to a decrease in temperature rather than in concentrations of organic phosphates. This is the first extensive study on freshwater turtle Hb isoforms, providing molecular evidence for adaptive changes in O2 transport associated with acclimation to severe hypoxia.
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