ArticleNucleic acids research2013
Structural insights into VirB-DNA complexes reveal mechanism of transcriptional activation of virulence genes.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneri.Nucleic acids research · 2026Article
- Remote Regulation by VirB, the Transcriptional Anti-Silencer of Shigella Virulence Genes, Provides Mechanistic Information.Molecular microbiology · 2025Article
- The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression.Nature communications · 2024Article
- VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor.Communications biology · 2023Article
- VirB, a key transcriptional regulator ofmBio · 2023Article
- Search for Origins of Anti-CRISPR Proteins by Structure Comparison.The CRISPR journal · 2023Article
- VirB, a key transcriptional regulator ofbioRxiv : the preprint server for biology · 2023Article
- Localized modulation of DNA supercoiling, triggered by the Shigella anti-silencer VirB, is sufficient to relieve H-NS-mediated silencing.Nucleic acids research · 2023Article
- Article
- Exploration of DNA processing features unravels novel properties of ICE conjugation in Gram-positive bacteria.Nucleic acids research · 2022Article
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- The Antiactivator of Type III Secretion, OspD1, Is Transcriptionally Regulated by VirB and H-NS from Remote Sequences in Shigella flexneri.Journal of bacteriology · 2020Article
- Virulence-Related Gene Distribution AmongInfection and drug resistance · 2020Article
- Existence of virulence genes in clinicalAnnals of translational medicine · 2019Article
- Article
- Insights into transcriptional silencing and anti-silencing in Shigella flexneri: a detailed molecular analysis of the icsP virulence locus.Molecular microbiology · 2018Article
- Low distribution of genes encoding virulence factors inGut pathogens · 2017Article
- Review
- Integrated circuits: how transcriptional silencing and counter-silencing facilitate bacterial evolution.Current opinion in microbiology · 2015Review
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Authors and funding
9 authors.
Funding
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Abstract
VirB activates transcription of virulence genes in Shigella flexneri by alleviating heat-stable nucleoid-structuring protein-mediated promoter repression. VirB is unrelated to the conventional transcriptional regulators, but homologous to the plasmid partitioning proteins. We determined the crystal structures of VirB HTH domain bound by the cis-acting site containing the inverted repeat, revealing that the VirB-DNA complex is related to ParB-ParS-like complexes, presenting an example that a ParB-like protein acts exclusively in transcriptional regulation. The HTH domain of VirB docks DNA major groove and provides multiple contacts to backbone and bases, in which the only specific base readout is mediated by R167. VirB only recognizes one half site of the inverted repeats containing the most matches to the consensus for VirB binding. The binding of VirB induces DNA conformational changes and introduces a bend at an invariant A-tract segment in the cis-acting site, suggesting a role of DNA remodeling. VirB exhibits positive cooperativity in DNA binding that is contributed by the C-terminal domain facilitating VirB oligomerization. The isolated HTH domain only confers partial DNA specificity. Additional determinants for sequence specificity may reside in N- or C-terminal domains. Collectively, our findings support and extend a previously proposed model for relieving heat-stable nucleoid-structuring protein-mediated repression by VirB.
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