Evidence map›Paper›PMID 23985698›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014

Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

Paul J Mintz, Kai-Wen Huang, Vikash Reebye, Georgios Nteliopoulos, Hong-Shiee Lai, Pal Sætrom, Noriyuki Kasahara, Steen Jensen, Madhava Pai, Myrtle Y A Gordon and 8 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 7 institutions in 6 countries.

Paul J Mintz1] Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK [2] Qatar Biomedical Research Institute, Education City, Doha, Qatar.
Kai-Wen Huang1] Department of Surgery & Hepatitis Research Center, National Taiwan University Hospital, Taipei City, Taiwan [2] Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, Taiwan.
Vikash ReebyeDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
Georgios NteliopoulosDepartment of Haematology, Faculty of Medicine, Imperial College London, London, UK.
Hong-Shiee Lai1] Department of Surgery & Hepatitis Research Center, National Taiwan University Hospital, Taipei City, Taiwan [2] Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, Taiwan.
Pal Sætrom1] Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway [2] Department of Computer and Information Science, Norwegian University of Science and Technology, Trondheim, Norway.
Noriyuki KasaharaDepartment of Medicine, UCLA School of Medicine, Los Angeles, California, USA.
Steen JensenDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
Madhava PaiDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
Myrtle Y A GordonDepartment of Haematology, Faculty of Medicine, Imperial College London, London, UK.
Stephen B MarleyDepartment of Haematology, Faculty of Medicine, Imperial College London, London, UK.
Rosemary BehanDepartment of Haematology, Faculty of Medicine, Imperial College London, London, UK.
Duncan R SpaldingDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
Abdelali HaoudiQatar Biomedical Research Institute, Education City, Doha, Qatar.
Mohamed M Emara1] Qatar Biomedical Research Institute, Education City, Doha, Qatar [2] Department of Virology, School of Veterinary Medicine, Cairo University, Cairo, Egypt.
Joanna NichollsDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
John J RossiDivision of Molecular Biology, Beckman Research Institute of City of Hope, Duarte, California, USA.
Nagy A HabibDepartment of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
Imperial College London · GBNational Taiwan University Hospital · TWQatar Foundation · QACairo University · EGCity of Hope · USNorwegian University of Science and Technology · NOUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34+ cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34+ cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34+ cells has the potential for therapeutic repair of damaged tissue in vivo.

Indexed as

AnimalsAntigens, CD34BiomarkersCell DeathCell LineCulture Media, ConditionedCulture Media, Serum-FreeCytokinesHematopoietic Stem CellsHumansLiver RegenerationMalePrimary Cell CultureProtein Interaction MappingProtein Interaction MapsRatsAntigens, CD34BiomarkersCulture Media, ConditionedCulture Media, Serum-FreeCytokines

Identifiers

PMID23985698
PMCPMC3978788
OpenAlexW2004539187

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.