ArticleJournal of virology1990
Karyophilic properties of Semliki Forest virus nucleocapsid protein.
Article in Journal of virology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Multiomics Analysis of Arboviral Capsid Targets in Mosquitoes Reveals a Proviral Function of the Chromatin-Remodeling Brahma Complex.Molecular & cellular proteomics : MCP · 2026Article
- The sticky business of Alphavirus capsid-host interactions.Trends in microbiology · 2025Review
- The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication.Protein & cell · 2022Article
- Characterisation of the Semliki Forest Virus-host cell interactome reveals the viral capsid protein as an inhibitor of nonsense-mediated mRNA decay.PLoS pathogens · 2021Article
- ArthritogenicLife (Basel, Switzerland) · 2021Review
- Dynamic Nucleolar Targeting of Dengue Virus Polymerase NS5 in Response to Extracellular pH.Journal of virology · 2016Article
- A novel system for visualizing alphavirus assembly.Journal of virological methods · 2015Article
- Viruses and the nucleolus: the fatal attraction.Biochimica et biophysica acta · 2014Article
- Chikungunya virus capsid protein contains nuclear import and export signals.Virology journal · 2013Article
- Optimization of transfection methods for Huh-7 and Vero cells: A comparative study.Cytology and genetics · 2012Article
- Identification and characterization of a novel non-structural protein of bluetongue virus.PLoS pathogens · 2011Article
- Low temperature-dependent salmonid alphavirus glycoprotein processing and recombinant virus-like particle formation.PloS one · 2011Article
- Development of infectious cDNA clones of Salmonid alphavirus subtype 3.BMC research notes · 2010Article
- A five-amino-acid deletion of the eastern equine encephalitis virus capsid protein attenuates replication in mammalian systems but not in mosquito cells.Journal of virology · 2008Article
- RNA viruses: hijacking the dynamic nucleolus.Nature reviews. Microbiology · 2007Review
- Nuclear localization of Japanese encephalitis virus core protein enhances viral replication.Journal of virology · 2005Article
- The interaction of animal cytoplasmic RNA viruses with the nucleus to facilitate replication.Virus research · 2003Review
- Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2.The EMBO journal · 1998Article
- The alphaviruses: gene expression, replication, and evolution.Microbiological reviews · 1994Review
- Two nucleolar targeting signals present in the N-terminal part of Semliki Forest virus capsid protein.Archives of virology · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semliki Forest virus capsid (C) protein molecules (Mr, 33,000) can be introduced efficiently into the cytoplasm of various target cells by electroporation, liposome, and erythrocyte ghost-mediated delivery (M. Elgizoli, Y. Dai, C. Kempf, H. Koblet, and M.R. Michel, J. Virol. 63:2921-2928, 1989). Here, we show that the transferred C protein molecules partition rapidly from the cytosolic compartment into the nucleus. Transport of the C protein molecules into the nucleus was reversibly arrested by metabolic inhibitors, indicating that the transfer process is energy dependent. Fractionation of isolated nuclei revealed that the delivered C protein preferentially associates with the nucleoli. This finding was confirmed by morphological studies, showing that in an in vitro system containing ATP isolated nuclei rapidly accumulated rhodamine-labeled C protein in their nucleoli. Furthermore, in this assay system, the lectin wheat germ agglutinin prevented transfer of C protein through nuclear pores. These results are in agreement with our observation that nucleoli contain measurable amounts of newly synthesized C protein as early as 5 h after infection of cells with SFV. Thereafter, nucleolar-associated C protein increased progressively during the course of infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.