ArticleAMB Express2013
Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation.
Article in AMB Express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Dinuclear Copper(II) Complexes of 2,6-Bis[(International journal of molecular sciences · 2025Article
- Leveraging bacterial laccases to facilitate the decomposition of xenobiotic compounds: a review.3 Biotech · 2024Review
- Lignin degradation by a novel thermophilic and alkaline yellow laccase fromMicrobiology spectrum · 2024Article
- Laccase Production fromMicroorganisms · 2023Article
- Deconstruction of Lignin: From Enzymes to Microorganisms.Molecules (Basel, Switzerland) · 2021Review
- Adaptive Enrichment of a Thermophilic Bacterial Isolate for Enhanced Enzymatic Activity.Microorganisms · 2020Article
- Recent Development of Extremophilic Bacteria and Their Application in Biorefinery.Frontiers in bioengineering and biotechnology · 2020Review
- Laccase: a multi-purpose biocatalyst at the forefront of biotechnology.Microbial biotechnology · 2017Review
- Purification and immobilization of laccase fromJournal, genetic engineering & biotechnology · 2017Article
- How to enjoy laccases.Cellular and molecular life sciences : CMLS · 2015Review
- Actinomycetes: A Source of Lignocellulolytic Enzymes.Enzyme research · 2015Review
- Thermophilic and alkaliphilic Actinobacteria: biology and potential applications.Frontiers in microbiology · 2015Review
- Characterization of an alkali- and halide-resistant laccase expressed in E. coli: CotA from Bacillus clausii.PloS one · 2014Article
- Structure-based rational design to enhance the solubility and thermostability of a bacterial laccase Lac15.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laccases are diphenol oxidases that have numerous applications to biotechnological processes. In this study, the laccase was produced from the thermophilic actinomycetes, Thermobifida fusca BCRC 19214. After 36 h of fermentation in a 5-liter fermentor, the culture broth accumulated 4.96 U/ml laccase activity. The laccase was purified 4.64-fold as measured by specific activity from crude culture filtrate by ultrafiltration concentration, Q-Sepharose FF and Sephacryl™ S-200 column chromatography. The overall yield of the purified enzyme was 7.49%. The molecular mass of purified enzyme as estimated by SDS-PAGE and by gel filtration on Sephacryl™ S-200 was found to be 73.3 kDa and 24.7 kDa, respectively, indicating that the laccase from T. fusca BCRC 19214 is a trimer. The internal amino acid sequences of the purified laccase, as determined by LC-MS/MS, had high homology with a superoxide dismutase from T. fusca YX. Approximately 95% of the original activity remained after treatment at 50°C for 3 h. and approximately 75% of the original activity remained after treatment at pH 10.0 for 24 h. This laccase could oxidize dye intermediates, especially 2,6-dimethylphenylalanine and p-aminophenol, to produce coloring. This is the first report on laccase properties from thermophilic actinomycetes. These properties suggest that this newly isolated laccase has potential for specific industrial applications.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.