ArticleParasites & vectors2013
Real-time PCR assay in differentiating Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii infections in Orang Asli settlements in Malaysia.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Prevalence and risk factors of intestinal protozoal infections among patients in Malaysia: A systematic review and meta-analysis.PloS one · 2025Pooled it
- First molecular detection and phylogenetic characterization of Balantioides coli in lowland tapir (Tapirus terrestris).Parasitology research · 2026Article
- The Taxonomy of the GenusPathogens (Basel, Switzerland) · 2026Review
- Evaluation of Each ThreeMicroorganisms · 2025Article
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- Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil.Pathogens (Basel, Switzerland) · 2025Article
- Evaluation of IL-17 and IL-35 in patients with giardiasis in Thi-Qar province, Iraq.Journal of medicine and life · 2022Article
- Development of a salivary IgA detection method for accurate diagnosis of amebiasis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Presence and genetic diversity of enteric protists in captive and semi-captive non-human primates in côte d'Ivoire, Sierra Leone, and Peru.International journal for parasitology. Parasites and wildlife · 2022Article
- Epidemiology of clinically relevant Entamoeba spp. (E. histolytica/dispar/moshkovskii/bangladeshi): A cross sectional study from North India.PLoS neglected tropical diseases · 2021Article
- Article
- The epidemiology of amoebiasis in Thi-Qar Province, Iraq (2015-2020): differentiation of Entamoeba histolytica and Entamoeba dispar using nested and real-time polymerase chain reaction.Epidemiology and health · 2021Article
- Genetic Diversity and Gene Family Expansions in Members of the Genus Entamoeba.Genome biology and evolution · 2019Article
- Update on laboratory diagnosis of amoebiasis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Domiciliary Cockroaches as Carriers of Human Intestinal Parasites in Lagos Metropolis, Southwest Nigeria: Implications for Public Health.Journal of arthropod-borne diseases · 2018Article
- Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal TractClinical microbiology reviews · 2018Review
- COMPARISON OF MULTIPLEX SINGLE ROUND PCR AND MICROSCOPY IN DIAGNOSIS OF AMOEBIASIS.African journal of infectious diseases · 2018Article
- Entamoeba Species in South Africa: Correlations With the Host Microbiome, Parasite Burdens, and First Description of Entamoeba bangladeshi Outside of Asia.The Journal of infectious diseases · 2017Article
- TheCurrent tropical medicine reports · 2016Article
- Comparison of Various Methods in the Diagnosis of Entamoeba histolytica in Stool and Serum Specimens.The Eurasian journal of medicine · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAmebiasis caused by Entamoeba histolytica is the third leading cause of death worldwide. This pathogenic amoeba is morphologically indistinguishable from E. dispar and E. moshkovskii, the non-pathogenic species. Polymerase chain reaction is the current method of choice approved by World Health Organization. Real-time PCR is another attractive molecular method for diagnosis of infectious diseases as post-PCR analyses are eliminated and turnaround times are shorter. The present work aimed to compare the results of Entamoeba species identification using the real-time assay against the established nested PCR method.
methodsIn this study, a total of 334 human faecal samples were collected from different Orang Asli settlements. Faecal samples were processed by direct wet smear and formalin ethyl acetate concentration methods followed by iodine staining and was microscopically examined for Entamoeba species and other intestinal parasites. Microscopically positive samples were then subject to nested PCR and real-time PCR.
resultsThe overall prevalence of Entamoeba infection was 19.5% (65/334). SK Posh Piah recorded highest Entamoeba prevalence (63.3%) while Kampung Kemensah had the lowest prevalence (3.7%) of Entamoeba. Microscopically positive samples were then tested by real-time PCR and nested PCR for the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii infection. Real-time PCR showed higher Entamoeba detection (86.2%) compared to nested PCR (80%), although the McNemar test value showed no significant difference between the two methods (p = 0.221).
conclusionsThis study is the first in Malaysia to report the use of real-time PCR in identifying and differentiating the three Entamoeba infections. It is also proven to be more effective compared to the conventional nested PCR molecular method.
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