ArticleBMC genomics2013
The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
- Targeting Toll-like Receptor 2: synthetic diacylated lipopeptides polarize equine macrophages towards a pro-inflammatory phenotype.Frontiers in immunology · 2026Article
- Breed Varieties of Pigs for Disease Resistance and Susceptibility to Seneca Valley Virus Infection.International journal of molecular sciences · 2025Article
- Immunopeptidomics ofBiology · 2024Article
- Phenotypic characterisation of bovine alveolar macrophages reveals two major subsets with differential expression of CD163.Scientific reports · 2024Article
- The African Swine Fever Virus Virulence Determinant DP96R Suppresses Type I IFN Production Targeting IRF3.International journal of molecular sciences · 2024Article
- Heterogeneity of Phenotypic and Functional Changes to Porcine Monocyte-Derived Macrophages Triggered by Diverse Polarizing Factors In Vitro.International journal of molecular sciences · 2023Article
- Goat milk extracellular vesicles: immuno-modulation effects on porcine monocyte-derived macrophagesFrontiers in immunology · 2023Article
- Article
- Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses.Pathogens (Basel, Switzerland) · 2022Article
- Cadmium and wild boar: Environmental exposure and immunological impact on macrophages.Toxicology reports · 2022Article
- The 3D nuclear conformation of the major histocompatibility complex changes upon cell activation both in porcine and human macrophages.BMC molecular and cell biology · 2021Article
- Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide.Vaccines · 2021Article
- Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing.Frontiers in genetics · 2021Article
- Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.Veterinary immunology and immunopathology · 2020Article
- Modulation of Type I Interferon System by African Swine Fever Virus.Pathogens (Basel, Switzerland) · 2020Article
- Comparison of Macrophage Responses to African Swine Fever Viruses Reveals that the NH/P68 Strain is Associated with Enhanced Sensitivity to Type I IFN and Cytokine Responses from Classically Activated Macrophages.Pathogens (Basel, Switzerland) · 2020Article
- Changes in H3K27ac at Gene Regulatory Regions in Porcine Alveolar Macrophages Following LPS or PolyIC Exposure.Frontiers in genetics · 2020Article
- Transcriptomic Analysis of Rat Macrophages.Frontiers in immunology · 2020Article
- Species-Specificity of Transcriptional Regulation and the Response to Lipopolysaccharide in Mammalian Macrophages.Frontiers in cell and developmental biology · 2020Article
- Functional Annotation of the Transcriptome of the Pig,Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
backgroundThe draft genome of the domestic pig (Sus scrofa) has recently been published permitting refined analysis of the transcriptome. Pig breeds have been reported to differ in their resistance to infectious disease. In this study we examine whether there are corresponding differences in gene expression in innate immune cells
resultsWe demonstrate that macrophages can be harvested from three different compartments of the pig (lungs, blood and bone-marrow), cryopreserved and subsequently recovered and differentiated in CSF-1. We have performed surface marker analysis and gene expression profiling on macrophages from these compartments, comparing twenty-five animals from five different breeds and their response to lipopolysaccharide. The results provide a clear distinction between alveolar macrophages (AM) and monocyte-derived (MDM) and bone-marrow-derived macrophages (BMDM). In particular, the lung macrophages express the growth factor, FLT1 and its ligand, VEGFA at high levels, suggesting a distinct pathway of growth regulation. Relatively few genes showed breed-specific differential expression, notably CXCR2 and CD302 in alveolar macrophages. In contrast, there was substantial inter-individual variation between pigs within breeds, mostly affecting genes annotated as being involved in immune responses.
conclusionsPig macrophages more closely resemble human, than mouse, in their set of macrophage-expressed and LPS-inducible genes. Future research will address whether inter-individual variation in macrophage gene expression is heritable, and might form the basis for selective breeding for disease resistance.
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