ArticleInternational journal of biological sciences2013
Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae).
Article in International journal of biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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105 citing papers in PubMed.
- Gluconeogenic compensation renders glycogen phosphorylase non-essential as an insecticidal target ineLife · 2026Article
- Identification of condition-specific optimal reference genes for qPCR data normalisation in pink bollworm Pectinophora gossypiella (Saunders).Molecular biology reports · 2026Article
- Selection and Validation of Stable Reference Genes for RT-qPCR inInternational journal of molecular sciences · 2026Article
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- Exploration of real-time fluorescence quantitative PCR by analyzing effective reference genes in Galleria mellonella (Lepidoptera: Pyralidae).Journal of insect science (Online) · 2025Article
- Stability Evaluation of Reference Genes inInsects · 2025Article
- Reference Genes for Expression Analyses by qRT-PCR inBiology · 2025Article
- Evaluation of stable reference genes for RT-qPCR analysis in Antheraea assamensis (Lepidoptera: Saturniidae) during pathogenic infections.Molecular biology reports · 2025Article
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- Identification of potential anti aging drugs and targets in chronic kidney disease.Scientific reports · 2025Article
- Adult Diel Locomotor Behaviour in the Agricultural PestInsects · 2025Article
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- MicroRNA Targets PAP1 to Mediate Melanization inInternational journal of molecular sciences · 2024Article
- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
- Identification of specific reference gene for normalization of RT-qPCR data in rhythmic gene expression studies of the effect of developmental hormone antagonist in postembryonic development inFrontiers in insect science · 2024Article
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- Selection and validation of optimal reference genes for RT-qPCR analyses inFrontiers in physiology · 2023Article
- Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR inFrontiers in physiology · 2023Article
45 more citing papers are in PubMed but not listed here.
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9 authors.
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Abstract
Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires an appropriate normalization strategy to control for variation among samples. The best option is to compare the mRNA level of a target gene with that of reference gene(s) whose expression level is stable across various experimental conditions. In this study, expression profiles of eight candidate reference genes from the diamondback moth, Plutella xylostella, were evaluated under diverse experimental conditions. RefFinder, a web-based analysis tool, integrates four major computational programs including geNorm, Normfinder, BestKeeper, and the comparative ΔCt method to comprehensively rank the tested candidate genes. Elongation factor 1 (EF1) was the most suited reference gene for the biotic factors (development stage, tissue, and strain). In contrast, although appropriate reference gene(s) do exist for several abiotic factors (temperature, photoperiod, insecticide, and mechanical injury), we were not able to identify a single universal reference gene. Nevertheless, a suite of candidate reference genes were specifically recommended for selected experimental conditions. Our finding is the first step toward establishing a standardized qRT-PCR analysis of this agriculturally important insect pest.
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