ArticleClinica chimica acta; international journal of clinical chemistry2013
Analytical characterization and clinical evaluation of an enzyme-linked immunosorbent assay for measurement of afamin in human plasma.
Article in Clinica chimica acta; international journal of clinical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 51 citations in OpenAlex.
- Effect of a short-term vitamin E supplementation on oxidative stress in infertile PCOS women under ovulation induction: a retrospective cohort study.BMC women's health · 2020Trial
- Multi-omic characterization of the sow colostrum and milk microbiome and proteome.Microbial genomics · 2026Article
- Efficiency of afamin and fibroblast growth factor 21 for early prediction of gestational diabetes mellitus.Scientific reports · 2026Article
- Higher afamin concentrations are associated with higher fatty liver indices: Population-based KORA F4/FF4 study.European journal of clinical investigation · 2025Article
- Comparative Analysis of Afamin, Asprosin, and Pentraxin 3 Levels in Women with PCOS and Women with Unexplained Infertility.Medical science monitor : international medical journal of experimental and clinical research · 2025Article
- Article
- Serum afamin and its implications in adult growth hormone deficiency: a prospective GH-withdrawal study.Frontiers in endocrinology · 2024Article
- AFM negatively regulates the infiltration of monocytes to mediate sepsis-associated acute kidney injury.Frontiers in immunology · 2023Article
- Emerging Protein Biomarkers for the Diagnosis or Prediction of Gestational Diabetes-A Scoping Review.Journal of clinical medicine · 2021Article
- Serum Afamin a Novel Marker of Increased Hepatic Lipid Content.Frontiers in endocrinology · 2021Observational
- Afamin predicts gestational diabetes in polycystic ovary syndrome patients preconceptionally.Endocrine connections · 2019Article
- Proteomics and multivariate modelling reveal sex-specific alterations in distinct regions of human carotid atheroma.Biology of sex differences · 2018Article
- Afamin: an early predictor of preeclampsia.Archives of gynecology and obstetrics · 2018Article
- Is Afamin a novel biomarker for gestational diabetes mellitus? A pilot study.Reproductive biology and endocrinology : RB&E · 2018Article
- The N14 anti-afamin antibody Fab: a rare VActa crystallographica. Section D, Structural biology · 2016Article
- Low Mass Blood Peptides Discriminative of Inflammatory Bowel Disease (IBD) Severity: A Quantitative Proteomic Perspective.Molecular & cellular proteomics : MCP · 2016Article
- Diagnostic and prognostic serum marker of cholangiocarcinoma (Review).Oncology letters · 2015Article
- Afamin serum concentrations are associated with insulin resistance and metabolic syndrome in polycystic ovary syndrome.Reproductive biology and endocrinology : RB&E · 2014Article
- Serum concentrations of afamin are elevated in patients with polycystic ovary syndrome.Endocrine connections · 2014Article
- The vitamin E-binding protein afamin increases in maternal serum during pregnancy.Clinica chimica acta; international journal of clinical chemistry · 2014Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundComparative proteomics has recently identified afamin, the newest member of the albumin gene family, as a potential biomarker for ovarian cancer. The aim of this study was the analytical and clinical evaluation of a sandwich enzyme-linked immunosorbent assay for the determination of afamin in human plasma.
methodsWe evaluated precision, linearity, and detection limit of the assay, analyte stability and biological variability, determined reference values and quantified afamin concentrations in various diseases.
resultsWithin-run and total coefficients of variation were <10%. The method was linear across the tested measurement range. Detection limit was 7 mg/L for the assay. The analyte was stable for 24 h at room temperature, for 48 h at 4°C, and for at least one year at -20°C and -80°C. The reference change value for healthy individuals was 24%. Age- and sex-independent reference values in healthy blood donors were 45-99 mg/L (median 68 mg/L). In the clinical assay evaluation afamin plasma concentrations were modestly decreased in patients with heart failure. Patients with pneumonia or sepsis exhibited markedly decreased afamin plasma concentrations. However, patients with chronic renal disease or chronic obstructive pulmonary disease showed no difference in afamin plasma concentrations as compared to healthy individuals. Correlation analyses revealed an inverse association between afamin and inflammatory biomarkers.
conclusionsThe afamin assay meets quality specifications for laboratory medicine. The results of the clinical assay evaluation revealed novel insights with respect to afamin as a potential negative acute phase protein and should encourage further studies.
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