ArticleG3 (Bethesda, Md.)2013
Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases.
Article in G3 (Bethesda, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed.
- Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.Plant molecular biology · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- CRISPR/Cas9-mediated genome editing technique to control fall armyworm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Crop bioengineering via gene editing: reshaping the future of agriculture.Plant cell reports · 2024Review
- TALE-based organellar genome editing and gene expression in plants.Plant cell reports · 2024Review
- Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021Review
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects.International journal of molecular sciences · 2021Review
- An inducible genome editing system for plants.Nature plants · 2020Article
- Review
- Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing.Rice (New York, N.Y.) · 2020Article
- Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9.Frontiers in plant science · 2020Review
- Development of an Agrobacterium-delivered CRISPR/Cas9 system for wheat genome editing.Plant biotechnology journal · 2019Article
- Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants.Frontiers in plant science · 2019Review
- ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: A disconnect between somatic and germinal cells.Journal of genetics and genomics = Yi chuan xue bao · 2018Article
- New breeding technique "genome editing" for crop improvement: applications, potentials and challenges.3 Biotech · 2018Review
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Article
- CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering.Genetics research international · 2018Review
- A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.The Plant cell · 2017Article
- CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Custom TAL effector nucleases (TALENs) are increasingly used as reagents to manipulate genomes in vivo. Here, we used TALENs to modify the genome of the model plant, Arabidopsis thaliana. We engineered seven TALENs targeting five Arabidopsis genes, namely ADH1, TT4, MAPKKK1, DSK2B, and NATA2. In pooled seedlings expressing the TALENs, we observed somatic mutagenesis frequencies ranging from 2-15% at the intended targets for all seven TALENs. Somatic mutagenesis frequencies as high as 41-73% were observed in individual transgenic plant lines expressing the TALENs. Additionally, a TALEN pair targeting a tandemly duplicated gene induced a 4.4-kb deletion in somatic cells. For the most active TALEN pairs, namely those targeting ADH1 and NATA2, we found that TALEN-induced mutations were transmitted to the next generation at frequencies of 1.5-12%. Our work demonstrates that TALENs are useful reagents for achieving targeted mutagenesis in this important plant model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.