ArticleG3 (Bethesda, Md.)2013
Targeted deletion and inversion of tandemly arrayed genes in Arabidopsis thaliana using zinc finger nucleases.
Article in G3 (Bethesda, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
- Research progress in gene editing of Brassicaceae crops.Biodesign research · 2026Review
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Chromosome engineering points to the cis-acting mechanism of chromosome arm-specific telomere length setting and robustness of plant phenotype, chromatin structure and gene expression.The Plant journal : for cell and molecular biology · 2025Article
- Genomic view of heavy-ion-induced deletions associated with distribution of essential genes inFrontiers in plant science · 2024Article
- Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome.Frontiers in plant science · 2024Article
- Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants.Nature communications · 2023Article
- Multiplex Editing of theThe CRISPR journal · 2023Article
- Multiplex Genome-Editing Technologies for Revolutionizing Plant Biology and Crop Improvement.Frontiers in plant science · 2021Review
- Development of multiplex genome editing toolkits for citrus with high efficacy in biallelic and homozygous mutations.Plant molecular biology · 2020Article
- Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing.Rice (New York, N.Y.) · 2020Article
- From gene editing to genome engineering: restructuring plant chromosomes via CRISPR/Cas.aBIOTECH · 2020Review
- Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019Review
- Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants.Frontiers in plant science · 2019Review
- Zinc finger nuclease-mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum) using a DNA repair template.Plant biotechnology journal · 2018Article
- CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing.Frontiers in plant science · 2017Review
- Genetically modified (GM) crops: milestones and new advances in crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Review
- Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.Plant cell reports · 2016Article
- Genome engineering and plant breeding: impact on trait discovery and development.Plant cell reports · 2016Review
- Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut.Plant biotechnology journal · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Tandemly arrayed genes (TAGs) or gene clusters are prevalent in higher eukaryotic genomes. For example, approximately 17% of genes are organized in tandem in the model plant Arabidopsis thaliana. The genetic redundancy created by TAGs presents a challenge for reverse genetics. As molecular scissors, engineered zinc finger nucleases (ZFNs) make DNA double-strand breaks in a sequence-specific manner. ZFNs thus provide a means to delete TAGs by creating two double-strand breaks in the gene cluster. Using engineered ZFNs, we successfully targeted seven genes from three TAGs on two Arabidopsis chromosomes, including the well-known RPP4 gene cluster, which contains eight resistance (R) genes. The resulting gene cluster deletions ranged from a few kb to 55 kb with frequencies approximating 1% in somatic cells. We also obtained large chromosomal deletions of ~9 Mb at approximately one tenth the frequency, and gene cluster inversions and duplications also were achieved. This study demonstrates the ability to use sequence-specific nucleases in plants to make targeted chromosome rearrangements and create novel chimeric genes for reverse genetics and biotechnology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.