ArticleMolecular genetics and genomics : MGG2013
The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress.
Article in Molecular genetics and genomics : MGG, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- De novo transcriptome and tissue-specific gene expression analysis in three types of barley following exploring the mechanism of quercetin content in purple barley (Hordeum vulgare).BMC plant biology · 2026Article
- Identification of reference genes for gene expression assessment in Avena sativa under biotic stress triggered by Blumeria graminis.Scientific reports · 2024Article
- Selection of Reference Genes for Expression Normalization by RT-qPCR inCurrent issues in molecular biology · 2024Article
- Validation of Reference Genes in a Population of BlueberryPlants (Basel, Switzerland) · 2022Article
- Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.).Scientific reports · 2022Article
- Characterization of Siccibacter sp. Strain C2 a Novel Rhizobacterium that Enhances Tolerance of Barley to Salt Stress.Current microbiology · 2022Article
- Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions inFrontiers in genetics · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
- Genome-wide identification and expression profile under abiotic stress of the barley non-specific lipid transfer protein gene family and its Qingke Orthologues.BMC genomics · 2021Article
- Validation of Reference Genes for Studying Different Abiotic Stresses in Oat (Plants (Basel, Switzerland) · 2021Article
- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress.PloS one · 2018Article
- Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.Scientific reports · 2017Article
- Reference genes for normalization of qPCR assays in sugarcane plants under water deficit.Plant methods · 2017Article
- Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress.Plant methods · 2017Article
- Reliable reference genes for normalization of gene expression data in tea plants (Camellia sinensis) exposed to metal stresses.PloS one · 2017Article
- Article
- Selection of Reference Genes for Normalization of MicroRNA Expression by RT-qPCR in Sugarcane Buds under Cold Stress.Frontiers in plant science · 2016Article
- Endophytic colonization of barley (Hordeum vulgare) roots by the nematophagous fungus Pochonia chlamydosporia reveals plant growth promotion and a general defense and stress transcriptomic response.Journal of plant research · 2015Article
- Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
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Authors and funding
8 authors.
Funding
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Abstract
Drought and low temperature are the two most significant causes of abiotic stress in agricultural crops and, therefore, they pose considerable challenges in plant science. Hence, it is crucial to study response mechanisms and to select genes for identification signaling pathways that lead from stimulus to response. The assessment of gene expression is often attempted using real-time RT-PCR (qRT-PCR), a technique which requires a careful choice of reference gene(s) for normalization purpose. Here, we report a comparison of 13 potential reference genes for studying gene expression in the leaf and crown of barley seedlings subjected to low temperature or drought stress. All three currently available software packages designed to identify reference genes from qRT-PCR data (GeNorm, NormFinder and BestKeeper) were used to identify informative sets of up to three reference genes. Interestingly, the data obtained from the separate treatment of leaf and crown have led to the recommendations that HSP70 and S-AMD (and possibly HSP90) to be used as the reference genes for low-temperature stressed leaves, HSP90 and EF1α for low-temperature stressed crowns, cyclophilin and ADP-RF (and possibly ACT) for drought-stressed leaves, and EF1α and S-AMD for drought-stressed crowns. Our results have demonstrated that the gene expression can be highly tissue- or organ-specific in barley and have confirmed that reference gene choice is essential in qRT-PCR. The findings can also serve as guidelines for the selection of reference genes under different stress conditions and lay foundation for more accurate and widespread use of qRT-PCR in barley gene analysis.
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