ArticleJournal of visualized experiments : JoVE2013
4D multimodality imaging of Citrobacter rodentium infections in mice.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract.Infection and immunity · 2020Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.Virulence · 2018Review
- Citrobacter rodentium Relies on Commensals for Colonization of the Colonic Mucosa.Cell reports · 2017Article
- The Mouse Model of Infection with Citrobacter rodentium.Current protocols in immunology · 2017Article
- Model of Host-Pathogen Interaction Dynamics Links In Vivo Optical Imaging and Immune Responses.Infection and immunity · 2017Article
- Citrobacter rodentium mouse model of bacterial infection.Nature protocols · 2016Article
- Bioluminescent imaging reveals novel patterns of colonization and invasion in systemic Escherichia coli K1 experimental infection in the neonatal rat.Infection and immunity · 2015Article
- Practical Issues with the Use of Stem Cells for Cancer Gene Therapy.Stem cell reviews and reports · 2015Review
- Fermented dairy products modulate Citrobacter rodentium-induced colonic hyperplasia.The Journal of infectious diseases · 2014Article
- Citrobacter rodentium: infection, inflammation and the microbiota.Nature reviews. Microbiology · 2014Review
- zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish.Development (Cambridge, England) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
This protocol outlines the steps required to longitudinally monitor a bioluminescent bacterial infection using composite 3D diffuse light imaging tomography with integrated μCT (DLIT-μCT) and the subsequent use of this data to generate a four dimensional (4D) movie of the infection cycle. To develop the 4D infection movies and to validate the DLIT-μCT imaging for bacterial infection studies using an IVIS Spectrum CT, we used infection with bioluminescent C. rodentium, which causes self-limiting colitis in mice. In this protocol, we outline the infection of mice with bioluminescent C. rodentium and non-invasive monitoring of colonization by daily DLIT-μCT imaging and bacterial enumeration from feces for 8 days. The use of the IVIS Spectrum CT facilitates seamless co-registration of optical and μCT scans using a single imaging platform. The low dose μCT modality enables the imaging of mice at multiple time points during infection, providing detailed anatomical localization of bioluminescent bacterial foci in 3D without causing artifacts from the cumulative radiation. Importantly, the 4D movies of infected mice provide a powerful analytical tool to monitor bacterial colonization dynamics in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.